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  • Description:
  • IFN‐γ induces the cytotoxic function of IL‐4 DCs but not IL‐15 DCs. (A) 4T1 tumor cells were cultured for 48 h with untreated (NT), LPS‐ (0.1 μg/mL) or IFN‐γ‐ (5 ng/mL) activated day 6 CD11c+ IL‐4 DCs or IL‐15 DCs (DC: tumor cell ratio = 5:1) and tumor cell survival was determined using a Crystal Violet assay. NS, Nonsignificant difference when compared with tumor cells cultured with untreated (NT) IL‐15 DCs; *p < 0.001, **p < 0.002 compared with tumor cells cultured with the corresponding untreated (NT) DC group, Student's t‐test. (B) Tumor cells were cultured as in (A), but at the indicated tumor cell: DC ratios. (C and D) Tumor cells were cultured with DCs activated as indicated (High Mobility Goup Box‐1, HMGB1; anti‐CD40, aCD40; Apoptotic tumor cells, Apo; Necrotic tumor cells, Nec) and tumor cell survival was determined. (A–D) Data are shown as mean ± SD from triplicate wells and are from one representative experiment out of three performed. (E) Determination of the type of tumor cell death induced by DCs. Tumor cells were cultured as in (A) and subsequently stained with Cell Trace Violet before being cocultured with IL‐4 DCs treated with LPS (+ IL‐4 DC (LPS)) or IFN‐γ (+ IL‐4 DC (IFN‐γ)) or with IL‐15 DCs treated with LPS (+ IL‐15 DC (LPS)). Cells were stained with PI and Annexin‐V and analyzed after gating on Cell Trace Violet‐positive cells. Data shown are representative of two independent experiments.
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • © 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
  • Asset Type:
  • Image
  • Asset Subtype:
  • Illustration
  • Image Orientation:
  • Portrait
  • Image Dimensions:
  • 931 x 1286
  • Image File Size:
  • 779 KB
  • Creator:
  • Neale T. Hanke, Collin J. LaCasse, Claire B. Larmonier, Darya Alizadeh, Malika Trad, Nona Janikashvili, Bernard Bonnotte, Emmanuel Katsanis, Nicolas Larmonier
  • Credit:
  • Hanke, N. T., LaCasse, C. J., Larmonier, C. B., Alizadeh, D., Trad, M., Janikashvili, N., Bonnotte, B., Katsanis, E., & Larmonier, N. (2014). PIAS1 and STAT‐3 impair the tumoricidal potential of IFN‐γ‐stimulated mouse dendritic cells generated with IL‐15. European Journal of Immunology, 44(8), 2489-2499. https://doi.org/10.1002/eji.201343803.
  • Collection:
  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Neale T. Hanke, Collin J. LaCasse, Claire B. Larmonier, Darya Alizadeh, Malika Trad, Nona Janikashvili, Bernard Bonnotte, Emmanuel Katsanis, Nicolas Larmonier
  • Article Copyright Year:
  • 2014
  • Publication Volume:
  • 44
  • Publication Issue:
  • 8
  • Publication Date:
  • 08/01/2014
  • DOI:
  • https://doi.org/10.1002/eji.201343803

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