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  • Description:
  • Effect of γ‐secretase inhibitors on AICD‐GVP and NICD‐GVP release in rescued cells expressing human wild‐type or mutated PS1 or PS2. (A) MEFs PSdKO rescued with PS1/PS2 wt/DA were cotransfected with C99‐GVP construct and reporter genes and were treated for 16 hrs with DAPT 250 nM, 1 μM, 10 μM or L‐685 10 μM, 20 μM. (B) Rescued cell lines were cotransfected with NotchΔE‐GVP construct and reporter genes and were treated as described in (A). Quantification of AICD‐GVP (A) and NICD‐GVP (B) release was performed by measurement of luciferase activity. Results (means ± S.E.M.) are represented as percentages of signals measured in non‐treated (NT) rPS1wt or rPS2wt cells, respectively. *P < 0.05, **P < 0.01, ***P < 0.001 versus corresponding NT cell type, n = 9 in three independent experiments.
  • License:
  • CC-BY
  • Rights Holder:
  • Rights Managed by John Wiley & Sons
  • License Rights Holder:
  • Copyright © 2018 John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine
  • Asset Type:
  • Image
  • Asset Subtype:
  • Image Orientation:
  • Landscape
  • Image Dimensions:
  • x
  • Image File Size:
  • 122 KB
  • Creator:
  • Serena Stanga, Céline Vrancx, Bernadette Tasiaux, Claudia Marinangeli, Helena Karlström, Pascal Kienlen‐Campard
  • Credit:
  • Stanga, S., Vrancx, C., Tasiaux, B., Marinangeli, C., Karlström, H., & Kienlen‐Campard, P. (2018). Specificity of presenilin‐1‐ and presenilin‐2‐dependent γ‐secretases towards substrate processing. Journal of Cellular and Molecular Medicine, 22(2), 823-833..
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  • Restrictions:
  • This image was published the terms of the Creative Commons Attribution (CC BY) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Permission is not required for this kind of use.
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Serena Stanga, Céline Vrancx, Bernadette Tasiaux, Claudia Marinangeli, Helena Karlström, Pascal Kienlen‐Campard
  • Article Copyright Year:
  • 2018
  • Publication Volume:
  • 22
  • Publication Issue:
  • 2
  • Publication Date:
  • 02/01/2018
  • DOI:

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