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  • Description:
  • Ghrelin decreases the amplitude of evoked GABAergic synaptic and induced‐muscimol responses in GHRH–eGFP neurons expressing the GHS‐R1a receptor. (A) Averaged GABAergic synaptic current traces recorded from one ghrelin‐responding neuron before (CTL, blue, n = 27 traces), during (GHRL, red, n = 17) and after (WASH, green, n = 4) 300 nm ghrelin perfusion, and then in the presence of 10 μm bicuculline (BICU, black, n = 9). Scale bar – 100 pA, 10 ms. (B) Averaged GABAergic synaptic current traces recorded from one ghrelin‐insensitive neuron before (CTL, blue, n = 26 traces), during (GHRL, red, n = 21) and after (WASH, green, n = 21) 300 nm ghrelin perfusion, and then in the presence of 10 μm bicuculline (BICU, black, n = 10). Scale bar – 100 pA, 10 ms. (C) Time plot of evoked GABAergic synaptic responses normalized to control in the presence of 300 nm ghrelin for both ghrelin‐insensitive (black squares, n = 24) and ghrelin‐responding (red diamonds, n = 29) neurons. The response is totally blocked by 10 μm bicuculline. Only 29 out of 37 ghrelin‐sensitive neurons are presented in the graph due to the loss of eight cells just before bicuculline application. (D1) Representative recordings of 100 μm muscimol‐induced responses of a GHRH–eGFP neuron in control medium (blue trace) and in the presence of 300 nm ghrelin (red trace). Scale bars – 50 pA, 30 s. (D2) Mean amplitude of 100 μm muscimol‐induced responses in control medium and in the presence of 300 nm ghrelin (n = 10, ***P < 0.001, paired Student’s t‐test). For interpretation of color references in figure legend, please refer to the Web version of this article.
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • © 2011 INSERM. European Journal of Neuroscience © 2011 Federation of European Neuroscience Societies and Blackwell Publishing Ltd
  • Asset Type:
  • Image
  • Asset Subtype:
  • Illustration
  • Image Orientation:
  • Landscape
  • Image Dimensions:
  • 567 x 562
  • Image File Size:
  • 46 KB
  • Creator:
  • Dan D. Feng, Seung‐Kwon Yang, Catherine Loudes, Axelle Simon, Tamara Al‐Sarraf, Michael Culler, Rodrigo Alvear‐Perez, Catherine Llorens‐Cortes, Chen Chen, Jacques Epelbaum, Robert Gardette
  • Credit:
  • Feng, D. D., Yang, S.-K., Loudes, C., Simon, A., Al‐Sarraf, T., Culler, M., Alvear‐Perez, R., Llorens‐Cortes, C., Chen, C., Epelbaum, J., & Gardette, R. (2011). Ghrelin and obestatin modulate growth hormone‐releasing hormone release and synaptic inputs onto growth hormone‐releasing hormone neurons. European Journal of Neuroscience, 34(5), 732-744. https://doi.org/10.1111/j.1460-9568.2011.07787.x.
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  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Dan D. Feng, Seung‐Kwon Yang, Catherine Loudes, Axelle Simon, Tamara Al‐Sarraf, Michael Culler, Rodrigo Alvear‐Perez, Catherine Llorens‐Cortes, Chen Chen, Jacques Epelbaum, Robert Gardette
  • Article Copyright Year:
  • 2011
  • Publication Volume:
  • 34
  • Publication Issue:
  • 5
  • Publication Date:
  • 09/01/2011
  • DOI:
  • https://doi.org/10.1111/j.1460-9568.2011.07787.x

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