Asset Details

  • Description:
  • Alternatively spliced IK isoforms are overexpressed in premalignant thymocytes from Notch3‐IC transgenic (N3‐ICtg) mice. (A) CD4+ and/or CD8+ subset distributions (documented by two‐color FCA) of thymocytes from 2‐week‐old wt and N3‐ICtg mice. The number in each quadrant indicates the percentage of total cells represented by the corresponding subset. (B) IK isoform expression profiles assessed by RT–PCR (upper panel) and Western blot (lower panel) analysis of whole thymocyte lysates from two wt and two N3‐ICtg mice. IK‐1 and IK2/3: DNA‐binding IK isoforms; IK‐dn: dominant‐negative IK spliced variants incapable of DNA binding. PCR products were analyzed by Southern blot using IK‐6 cDNA as probe to detect the specific sequences. mRNA expression was monitored along the exponential phase of amplification and normalized to β‐actin (β‐act). In Western blot assay, results were normalized to β‐tubulin (β‐tub). The data are representative of three similar experiments.
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • Copyright © 2013 Wiley Periodicals, Inc
  • Asset Type:
  • Image
  • Asset Subtype:
  • Figure
  • Image Orientation:
  • Portrait
  • Image Dimensions:
  • 343 x 614
  • Image File Size:
  • 87.5 KB
  • Creator:
  • Diana Bellavia, Marco Mecarozzi, Antonio F Campese, Paola Grazioli, Claudio Talora, Luigi Frati, Alberto Gulino, Isabella Screpanti
  • Credit:
  • Bellavia, D., Mecarozzi, M., Campese, A. F., Grazioli, P., Talora, C., Frati, L., Gulino, A., & Screpanti, I. (2007). Notch3 and the Notch3‐upregulated RNA‐binding protein HuD regulate Ikaros alternative splicing. The EMBO Journal, 26(6), 1670-1680..
  • Collection:
  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Diana Bellavia, Marco Mecarozzi, Antonio F Campese, Paola Grazioli, Claudio Talora, Luigi Frati, Alberto Gulino, Isabella Screpanti
  • Article Copyright Year:
  • 2007
  • Publication Volume:
  • 26
  • Publication Issue:
  • 6
  • Publication Date:
  • 03/21/2007
  • DOI:

Click on image to enlarge

Would you like to download this asset? Tell us more and we can generate a quote, license and downloadable file.

Add to Cart