Asset Details

Identifier:
jlb0766-fig-0004-m

Description:
TLR2 activation by F. tularensis does not require LBP and occurs through TLR2/TLR1 and TLR2/TLR6 heterodimers. HeLa cell lines expressing the indicated TLR constructs were transiently transfected with ELAM‐luciferase reporter construct, CMV‐CD14, and CMV‐β‐Gal (for normalization) and stimulated for 6 h with the indicated agonists. NF‐κB activation was measured by luciferase assay. (A) HeLa‐TLR2 and HeLa‐TLR4/MD‐2 are stable cell lines, and expression of TLR5 and TLR7/TLR8 was established via transient transfections. (B) Parental HeLa cells were transiently transfected with the indicated TLR chimeric constructs and stimulated with the indicated agonists, which were used at the following concentrations: LPS (1 ng/ml), crude PGN (10 μg/ml), FSL‐1 (10 ng/ml), MALP‐2 (10 ng/ml), Pam3Cys (2 μg/ml), flagellin (10 μg/ml), R848 (1 μg/ml), live or heat‐killed F. tularensis at MOIs of 500 and 50, and PMA (10 ng/ml). (C) Schematic illustration of the TLR chimeric constructs and their modality of signaling. On the left, transfected TLR2 heterodimerizes with endogenous TLR1 or TLR6 and signals in response to triacylated or diacylated lipopeptides. Center, Chimeric constructs transfected in isolation cannot signal as a result of absence of heterodimerization of extracellular or cytoplasmic regions. On the right, complementing chimeric construct pairs restore signaling by providing simultaneously extracellular and cytoplasmic regions that can heterodimerize.

License:
Rights Managed

Rights Holder:
John Wiley & Sons, Inc.

License Rights Holder:
© Society for Leukocyte Biology

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Image

Asset Subtype:
Chart/Graph

Image Orientation:
Landscape

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x

Image File Size:
246 KB

Creator:
Hanfen Li, Suba Nookala, Xiaowen R. Bina, James E. Bina, Fabio Re

Credit:
Li, H., Nookala, S., Bina, X. R., Bina, J. E., & Re, F. (2006). Innate immune response to Francisella tularensis is mediated by TLR2 and caspase‐1 activation. Journal of Leukocyte Biology, 80(4), 766-773. https://doi.org/10.1189/jlb.0406294.