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  • Description:
  • Inhibition of MVB–lysosome fusion inhibits delivery of EGF to LBPA‐positive lysosomes. HEp2 cells were loaded with HRP in preparation for crosslinking. Noncrosslinked cells treated without DAB/H2O2 were stimulated with fluorescent EGF at 37°C for 60 min (A). Cells that were crosslinked with DAB/H2O2 were stimulated with fluorescent EGF for 180 min at 37°C (B). Cells were fixed prior to permeabilisation and then stained with antibodies against LBPA and LAMP 1. Colocalisation between EGF and LBPA appears as yellow in the merged images, LBPA and LAMP 1 as magenta, and colocalisation between all three (examples shown by arrows) as white. Vacuoles staining for both EGF and LBPA in noncrosslinked cells always also stain for LAMP 1. Crosslinking of lysosomes abolishes both the majority of LBPA staining and any colocalisation between EGF and LBPA. Bars=5 μm.
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • Copyright © 2013 Wiley Periodicals, Inc
  • Asset Type:
  • Image
  • Asset Subtype:
  • Photo
  • Image Orientation:
  • Landscape
  • Image Dimensions:
  • 732 x 565
  • Image File Size:
  • 278 KB
  • Creator:
  • Ian J White, Lorna M Bailey, Minoo Razi Aghakhani, Stephen E Moss, Clare E Futter
  • Credit:
  • White, I. J., Bailey, L. M., Aghakhani, M. R., Moss, S. E., & Futter, C. E. (2006). EGF stimulates annexin 1‐dependent inward vesiculation in a multivesicular endosome subpopulation. The EMBO Journal, 25(1), 1-12..
  • Collection:
  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Ian J White, Lorna M Bailey, Minoo Razi Aghakhani, Stephen E Moss, Clare E Futter
  • Article Copyright Year:
  • 2006
  • Publication Volume:
  • 25
  • Publication Issue:
  • 1
  • Publication Date:
  • 01/11/2006
  • DOI:

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