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  • Description:
  • Cold‐induced VEGF gene expression in brown adipose tissue. C57Bl/6 wild‐type mice and UCP1‐ablated mice were first acclimated to thermoneutrality and then cold‐exposed at 4 °C for the times indicated, after which the animals were killed and interscapular brown adipose tissue was dissected out. Total RNA was isolated and mRNA levels were determined by Northern blot analysis, as described in Section 2. For VEGF mRNA quantification, all visible transcripts were analysed together. (A) Northern blot of RNA from brown adipose tissue from wild‐type mice (wt) and from UCP1‐ablated mice (−/−) showing signals for UCP1 mRNA (upper panel (wt) and the UCP1 minigene in the UCP1‐ablated mice (−/−)), VEGF mRNA (middle panel) and ethidium bromide staining of the gel visualized under UV‐light (lower panel). (B) VEGF mRNA levels in brown adipose tissue after the indicated times of cold exposure. Filled circles, wild‐type mice (wt); open circles, UCP1‐ablated mice (UCP1(−/−)). Values are means ± S.E. from four experiments performed as described in Section 2. The value for UCP1(−/−) 1 h was set to 100% in each experimental series. Asterisks indicate the level of statistical difference versus the start value (0 h) and hatches indicate the level of statistical difference between genotypes for each time of cold exposure.
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • © 2015 Federation of European Biochemical Societies
  • Asset Type:
  • Image
  • Asset Subtype:
  • Chart/Graph
  • Image Orientation:
  • Portrait
  • Image Dimensions:
  • 364 x 630
  • Image File Size:
  • 34.3 KB
  • Creator:
  • J. Magnus Fredriksson, Hideki Nikami, Jan Nedergaard
  • Credit:
  • Fredriksson, J. M., Nikami, H., Nedergaard, J., Cannon, B., Nedergaard, J., Trayhurn, P., Folkman, J., Rupnick, M. A., Panigrahy, D., Zhang, C., Dallabrida, S. M., Lowell, B. B., Langer, R., Folkman, J., Bråkenhielm, E., Cao, R., Gao, B., Angelin, B., Cannon, B., Parini, P., Cao, Y., Hausman, G. J., Richardson, R. L., Ferrara, N., Zhang, Q.-X., Magovern, C. J., Mack, C. A., Budenbender, K. T., Ko, W., Rosengart, T. K., Lolmede, K., Durand de Saint Front, V., Galitzky, J., Lafontan, M., Bouloumie, A., Fukumura, D., Ushiyama, A., Duda, D. G., Xu, L., Tam, J., Krishna, V., Chatterjee, K., Garkavtsev, I., Jain, R. K., Trayhurn, P., Wood, I. S., Rehnmark, S., Nedergaard, J., Nedergaard, J., Herron, D., Jacobsson, A., Rehnmark, S., Cannon, B., Bukowiecki, L. J., Collet, A. J., Follea, N., Guay, G., Jahjah, L., Foster, D. O., Frydman, M. L., Foster, D. O., Frydman, M. L., Smith, R. E., Horwitz, B. A., Asano, A., Morimatsu, M., Nikami, H., Yoshida, T., Saito, M., Fredriksson, J. M., Lindquist, J. M., Bronnikov, G. E., Nedergaard, J., Ashwell, M., Jennings, G., Richard, D., Stirling, D. M., Trayhurn, P., Enerbäck, S., Jacobsson, A., Simpson, E. M., Guerra, C., Yamashita, H., Harper, M.-E., Kozak, L. P., Golozoubova, V., Hohtola, E., Matthias, A., Jacobsson, A., Cannon, B., Nedergaard, J., Matthias, A., Ohlson, K. E. B., Fredriksson, J. M., Jacobsson, A., Nedergaard, J., Cannon, B., Bronnikov, G., Bengtsson, T., Kramarova, L., Golozoubova, V., Cannon, B., Nedergaard, J., Breier, G., Albrecht, U., Sterrer, S., Risau, W., Rehnmark, S., Néchad, M., Herron, D., Cannon, B., Nedergaard, J., Robinson, C. J., & Stringer, S. E. (2005). Cold‐induced expression of the VEGF gene in brown adipose tissue is independent of thermogenic oxygen consumption. FEBS Letters, 579(25), 5680-5684. https://doi.org/10.1016/j.febslet.2005.09.
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  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • J. Magnus Fredriksson, Hideki Nikami, Jan Nedergaard
  • Article Copyright Year:
  • 2005
  • Publication Volume:
  • 579
  • Publication Issue:
  • 25
  • Publication Date:
  • 10/24/2005
  • DOI:

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