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  • Description:
  • Detection of DCL3A and DCL3B genes in monocots and their phylogenetic relationships. (A) The phylogenetic analysis of the helicase‐C domains of rice, maize, Arabidopsis and poplar DCL3‐type genes, with the inclusion of their DCL1 counterparts to root the tree. The analysis was done in a similar way to that described in Fig. 2. (B) PCR analysis for the detection of DCL3A and DCL3B genes in a range of monocots using A‐ and B‐specific primer pairs. The product from the 3B primers were expected to be larger (∼600nt) than the product from the detection of DCL3A (∼500nts). Lanes 1 and 18: markers; lanes 2, 4, 6, 10, 14 and 16 DCL3A‐specific primer pairs; lanes 3, 5, 7, 11, 15 and 17 DCL3B‐specific primer pairs. Lanes 8 and 12 negative control 3A forward with 3B reverse primers; lanes 9 and 13 negative control 3B forward with 3A reverse primer pairs. Lanes 2 and 3 water control; lanes 4 and 5 rice DNA; lanes 6–9 Triticum DNA; lanes 10–13 barley DNA; lanes 14 and 15 maize DNA and lanes 16 and 17 Arabidopsis DNA. The results show the detection of DCL3A and DCL3B in all of the monocots DNA tested.
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • © 2015 Federation of European Biochemical Societies
  • Asset Type:
  • Image
  • Asset Subtype:
  • Illustration
  • Image Orientation:
  • Portrait
  • Image Dimensions:
  • 556 x 618
  • Image File Size:
  • 37.6 KB
  • Creator:
  • Rogerio Margis, Adriana F. Fusaro, Neil A. Smith, Shaun J. Curtin, John M. Watson, E. Jean Finnegan, Peter M. Waterhouse
  • Credit:
  • Margis, R., Fusaro, A. F., Smith, N. A., Curtin, S. J., Watson, J. M., Finnegan, E. J., Waterhouse, P. M., Finnegan, E. J., Matzke, M. A., Bartel, D. P., Bernstein, E., Caudy, A. A., Hammond, S. M., Hannon, G. J., Schauer, S. E., Jacobsen, S. E., Meinke, D. W., Ray, A., Catalanotto, C., Pallotta, M., ReFalo, P., Sachs, M. S., Vayssie, L., Macino, G., Cogoni, C., Lee, Y. S., Nakahara, K., Pham, J. W., Kim, K., He, Z., Sontheimer, E. J., Carthew, R. W., Liu, B., Li, X., Liu, C., Cao, S., Chu, C., Cao, X., Finnegan, E. J., Margis, R., Waterhouse, P. M., Park, W., Li, J., Song, R., Messing, J., Chen, X., Xie, Z., Johansen, L. K., Gustafson, A. M., Kasschau, K. D., Lellis, A. D., Zilberman, D., Jacobsen, S. E., Carrington, J. C., Gasciolli, V., Mallory, A. C., Bartel, D. P., Vaucheret, H., Xie, Z., Allen, E., Wilken, A., Carrington, J. C., Borsani, O., Zhu, J., Verslues, P. E., Sunkar, R., Zhu, J. K., Millar, A. A., Jacobsen, J. V., Ross, J., Helliwell, C. A., Poole, A. T., Scofield, G., Reid, J. B., Gubler, F., Thompson, J., Higgins, D., Gibson, T., Kumar, S., Tamura, K., Nei, M., Zdobnov, E. M., Apweiler, R., Golden, T. A., Schauer, S. E., Lang, J. D., Pien, S., Mushegian, A. R., Grossniklaus, U., Meinke, D. W., Ray, A., Zhang, H., Kolb, F. A., Jaskiewicz, L., Westhof, E., Filipowicz, W., Carmell, M. A., Hannon, G. J., Cosentino, G., Venkatesan, S., Serluca, F., Green, S., Mathews, M., Sonenberg, N., Patel, R., Stanton, P., McMillan, N., Williams, B., Sen, G., Hiraguri, A., Itoh, R., Kondo, N., Nomura, Y., Aizawa, D., Murai, Y., Koiwa, H., Seki, M., Shinozaki, K., Fukuhara, T., Meister, G., Tuschl, T., Sterck, L., Rombauts, S., Jansson, S., Sterky, F., Rouze, P., Van de Peer, Y., Blanc, G., Wolfe, K. H., Ma, J., Bennetzen, J. L., Hedges, S. B., Blair, J. E., Venturi, M. L., Shoe, J. L., Mochizuki, K., & Gorovsky, M. A. (2006). The evolution and diversification of Dicers in plants. FEBS Letters, 580(10), 2442-2450. https://doi.org/10.1016/j.febslet.2006.03.
  • Collection:
  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Rogerio Margis, Adriana F. Fusaro, Neil A. Smith, Shaun J. Curtin, John M. Watson, E. Jean Finnegan, Peter M. Waterhouse
  • Article Copyright Year:
  • 2006
  • Publication Volume:
  • 580
  • Publication Issue:
  • 10
  • Publication Date:
  • 05/01/2006
  • DOI:

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