Asset Details

  • Description:
  • Chemical cross‐linking of DevR. Left: western blot analysis of cross‐linked unphosphorylated (Unphos) and phosphorylated (Phos) WT DevR. Right: western blot of cross‐linked unphosphorylated and phosphorylated DevR∆α10. In the western blots, 2.5, 5 and 10 μg of protein were incubated at room temperature for 60 min in the presence or absence of 50 mm of acetyl phosphate to yield phosphorylated and unphosphorylated protein, respectively, followed by cross‐linking with 1% glutaraldehyde for 15 min at 37 °C. Ten microgrammes of uncross‐linked WT DevR and DevR∆α10 were used as controls for unphosphorylated and phosphorylated species. M, molecular weight marker. SDS/PAGE was performed under reducing conditions (loading dye with β‐mercaptoethanol). Western blots were probed using monoclonal anti‐polyhistidine peroxidase antibody.
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • Copyright © 2016 Federation of European Biochemical Societies
  • Asset Type:
  • Image
  • Asset Subtype:
  • Figure
  • Image Orientation:
  • Landscape
  • Image Dimensions:
  • 2128 x 936
  • Image File Size:
  • 1.51 MB
  • Creator:
  • Atul Vashist, D. Prithvi Raj, Umesh Datta Gupta, Rajiv Bhat, Jaya Sivaswami Tyagi
  • Credit:
  • Vashist, A., Prithvi Raj, D., Gupta, U. D., Bhat, R., & Tyagi, J. S. (2016). The α10 helix of DevR, the Mycobacterium tuberculosis dormancy response regulator, regulates its DNA binding and activity. The FEBS Journal, 283(7), 1286-1299..
  • Collection:
  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Atul Vashist, D. Prithvi Raj, Umesh Datta Gupta, Rajiv Bhat, Jaya Sivaswami Tyagi
  • Article Copyright Year:
  • 2016
  • Publication Volume:
  • 283
  • Publication Issue:
  • 7
  • Publication Date:
  • 04/01/2016
  • DOI:

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