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  • Description:
  • MSC‐induced and monocyte‐dependent Treg formation is mediated by CCL18. (A): CCL18 protein concentrations measured with ELISA at day 3 of the monocyte culture are increased when monocytes were stimulated with CM. Addition of an inhibitor of the TGF‐β1‐receptor did not reverse the effect of CM and recombinant TGF‐β1 did not induce CCL18 secretion by monocytes. (Data are means of five monocyte donors and two MSC donors; *, compared to control; *, p < .05; **, p < .01; #, compared to M‐CSF; #, p < .05; ##, p < .01). (B): Representative dotplots of expression of CD4 and CD25 on day 14‐PBMC from control cultures and from PBMC‐CM cocultures in the presence of neutralizing antibodies against CCL18. (C): The percentage of CD4+CD25hi T cells at day 14 is significantly lower in the population that was cultured during the first week in the presence of aCCL18 compared to PBMC that were cultured in control PBMC‐CM cultures (data are means ± SEM from two different PBMC. Statistical analysis was performed with a Student's t test; **, p < .01). (D): Hypothetical model of Treg formation induced by MSC, both the direct pathway via MSC‐derived TGF‐β1 and the indirect pathway via the modulation of monocytes are crucially involved in the MSC‐induced Treg formation. Abbreviations: CM, conditioned medium; IL, interleukin; MSC, multipotent stromal cell; M‐CSF, macrophage colony‐stimulating factor; TGFβ, transforming growth factor beta.
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • © AlphaMed Press
  • Asset Type:
  • Image
  • Asset Subtype:
  • Illustration
  • Image Orientation:
  • Portrait
  • Image Dimensions:
  • 896 x 1376
  • Image File Size:
  • 371 KB
  • Creator:
  • Sara M. Melief, Ellen Schrama, Martijn H. Brugman, Machteld M. Tiemessen, Martin J. Hoogduijn, Willem E. Fibbe, Helene Roelofs
  • Credit:
  • Melief, S. M., Schrama, E., Brugman, M. H., Tiemessen, M. M., Hoogduijn, M. J., Fibbe, W. E., & Roelofs, H. (2013). Multipotent stromal cells induce human regulatory T cells through a novel pathway involving skewing of monocytes toward anti‐inflammatory macrophages. STEM CELLS, 31(9), 1980-1991..
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  • Keywords:
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  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Sara M. Melief, Ellen Schrama, Martijn H. Brugman, Machteld M. Tiemessen, Martin J. Hoogduijn, Willem E. Fibbe, Helene Roelofs
  • Article Copyright Year:
  • 2013
  • Publication Volume:
  • 31
  • Publication Issue:
  • 9
  • Publication Date:
  • 09/01/2013
  • DOI:

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