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  • Description:
  • Silencing of Mgp levels decreases the lung prometastatic activity of OS cells. (A) Relative levels of Mgp expression in cells lentivirally‐transduced with two different shRNAs targeting different murine Mgp sequences, assessed by RT–qPCR. (B) (Upper panel) Western blot analysis of Mgp protein levels in shMgp‐1 cells (sh‐Mgp) as compared to control (scramble‐transduced) and rescued (sh‐Δ‐Mgp) cells obtained from sh‐Mgp cells after retroviral transduction with a mutated synonymous sequence unrecognized by sh‐Mgp; a positive control (293‐Mgp) was included after overexpressing Mgp‐myc in HEK293 cells; (bottom panel) Mgp expression levels were assessed by RT–qPCR in control, sh‐Mgp and rescued cells (sh‐Δ‐Mgp). (C) (Upper panel) Representative X‐ray images and microCT scans of hind limbs; (lower panel) quantification of osteolytic area in the hind limbs showing no statistical differences. (D) (Upper panel) bioluminescence quantification of the thoracic cavity in athymic nude animals (eight mice/group) after intratibial injection of control and shMgp‐myc P1.15 and rescue Mgp cells 15 days postinjection; (lower panel) representative images of bioluminescence images; scale bar indicates the minimum and maximum emission of bioluminescence in photons/s × cm2 × steradians. (E) (Upper panel) Quantification of the area and number of metastatic nodules/lung in H&E‐stained sections as assessed by image analysis; mean and standard error (SE) of the mean are represented; (lower panel) representative H&E‐stained lung sections; *p < 0.05, **p < 0.01
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • Copyright © 2016 Pathological Society of Great Britain and Ireland
  • Asset Type:
  • Image
  • Asset Subtype:
  • Illustration
  • Image Orientation:
  • Landscape
  • Image Dimensions:
  • x
  • Image File Size:
  • 977 KB
  • Creator:
  • Carolina Zandueta, Cristina Ormazábal, Naiara Perurena, Susana Martínez‐Canarias, Marta Zalacaín, Mikel San Julián, Agamemnon E Grigoriadis, Karmele Valencia, Francisco J Campos‐Laborie, Javier De Las Rivas, Silvestre Vicent, Ana Patiño‐García, Fernando Lecanda
  • Credit:
  • Zandueta, C., Ormazábal, C., Perurena, N., Martínez‐Canarias, S., Zalacaín, M., Julián, M. S., Grigoriadis, A. E., Valencia, K., Campos‐Laborie, F. J., Rivas, J. D. L., Vicent, S., Patiño‐García, A., & Lecanda, F. (2016). Matrix‐Gla protein promotes osteosarcoma lung metastasis and associates with poor prognosis. The Journal of Pathology, 239(4), 438-449..
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  • Keywords:
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  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Carolina Zandueta, Cristina Ormazábal, Naiara Perurena, Susana Martínez‐Canarias, Marta Zalacaín, Mikel San Julián, Agamemnon E Grigoriadis, Karmele Valencia, Francisco J Campos‐Laborie, Javier De Las Rivas, Silvestre Vicent, Ana Patiño‐García, Fernando Lecanda
  • Article Copyright Year:
  • 2016
  • Publication Volume:
  • 239
  • Publication Issue:
  • 4
  • Publication Date:
  • 08/01/2016
  • DOI:

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