Asset Details

  • Description:
  • Inhibition on the PI3K/Akt pathway attenuates hypoxia‐inducible factor‐2α (HIF‐2α) upregulation triggered by stearoyl–CoA desaturase‐1 (SCD1) overexpression. (A) Scramble sequence or siRNA against SCD1 (shSCD1) was transfected into 786‐0 renal cancer cells. After puromycin selection (1 μg/mL for 14 days), the cells were exposed to hypoxia for 16 h. The protein levels for SCD1 and HIF‐2α were analyzed with Western blot. Cells transfected with scrambled sequence and cultured under normoxic conditions were taken as control (Ctrl). (B) SCD1‐expressing plasmids (2 μg) were transiently transfected into 786‐0 cells. The protein levels for SCD1 and HIF‐2α were analyzed with Western blot after 48 h. Cells transfected with empty vector (EV; 2 μg) were taken as control. (C) SCD1‐expressing plasmid (2 μg) was transfected into 786‐0 cells. After 24 h, the cells were replaced with medium containing PI3K/Akt pathway inhibitor Ly294002 (25 μM) or vehicle (DMSO) and incubated for a further 24 h. The levels of HIF‐2α, SCD1, total Akt, and phosphorylated Akt were detected by Western blot. Cells transfected with empty vector (2 μg) and incubated with DMSO were taken as control (Ctrl).
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • © 2013 Japanese Cancer Association
  • Asset Type:
  • Image
  • Asset Subtype:
  • Figure
  • Image Orientation:
  • Portrait
  • Image Dimensions:
  • x
  • Image File Size:
  • 99.4 KB
  • Creator:
  • Yujian Zhang, Hui Wang, Jin Zhang, Jianwei Lv, Yiran Huang
  • Credit:
  • Zhang, Y., Wang, H., Zhang, J., Lv, J., & Huang, Y. (2013). Positive feedback loop and synergistic effects between hypoxia‐inducible factor‐2α and stearoyl–CoA desaturase‐1 promote tumorigenesis in clear cell renal cell carcinoma. Cancer Science, 104(4), 416-422..
  • Collection:
  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Yujian Zhang, Hui Wang, Jin Zhang, Jianwei Lv, Yiran Huang
  • Article Copyright Year:
  • 2013
  • Publication Volume:
  • 104
  • Publication Issue:
  • 4
  • Publication Date:
  • 04/01/2013
  • DOI:

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