Asset Details

  • Description:
  • Effects of stearoyl–CoA desaturase‐1 (SCD1) and hypoxia‐inducible factor‐2α (HIF‐2α) on apoptosis and colony formation ability. (A) Renal carcinoma 786‐0 cells were transfected with the following siRNA combinations. After selection, the protein levels for SCD1 and HIF‐2α were analyzed using Western blot. Lane 1, scrambled sequences for SCD1 and HIF‐2α (Ctrl); lane 2, siRNA against HIF‐2α (shHIF‐2α) + scrambled sequences for siRNA against SCD1 (shSCD1); lane 3, shSCD1 + scrambled sequences for shHIF‐2α; lane 4, shSCD1 + shHIF‐2α. (B) 786‐0 cells infected with various siRNA combinations were cultured for 72 h and subjected to TUNEL staining; DAPI was used to indicate the nuclei. At least 300 cells from randomly selected fields were counted and the percentage of TUNEL positively stained cells was calculated. (C) 786‐0 cells infected with siRNA were seeded at 1 × 105 in a 35‐mm dish in soft agar and cultured for 14 days for colony formation. *P < 0.05 for shSCD1 or shHIF‐2α versus Ctrl; **P < 0.05 for double shRNA versus shSCD1 or shHIF‐2α.
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • © 2013 Japanese Cancer Association
  • Asset Type:
  • Image
  • Asset Subtype:
  • Illustration
  • Image Orientation:
  • Portrait
  • Image Dimensions:
  • x
  • Image File Size:
  • 799 KB
  • Creator:
  • Yujian Zhang, Hui Wang, Jin Zhang, Jianwei Lv, Yiran Huang
  • Credit:
  • Zhang, Y., Wang, H., Zhang, J., Lv, J., & Huang, Y. (2013). Positive feedback loop and synergistic effects between hypoxia‐inducible factor‐2α and stearoyl–CoA desaturase‐1 promote tumorigenesis in clear cell renal cell carcinoma. Cancer Science, 104(4), 416-422..
  • Collection:
  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Yujian Zhang, Hui Wang, Jin Zhang, Jianwei Lv, Yiran Huang
  • Article Copyright Year:
  • 2013
  • Publication Volume:
  • 104
  • Publication Issue:
  • 4
  • Publication Date:
  • 04/01/2013
  • DOI:

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