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  • Description:
  • Expression of histochemical markers in spotted leaf 28 (spl28) mutants. (a) Detection of phenolic compounds in the leaves of wild‐type and spl28 mutant plants. Bright regions indicate the accumulation of autofluorescent protein. LM, lesion mimics. (b) Detection of callose in leaves of wild‐type and spl28 mutant plants. The accumulation of callose (deep‐blue color) is indicated by white arrows. For phenolic compounds and callose, the cleared leaves were observed by microscopy under UV light. (c) Cell death and reactive oxygen species (ROS) accumulation in wild‐type and spl28 mutant plants. Dead cells were detected by trypan blue staining in spl28 mutants (left panel) as areas of deep purple color in leaves with lesions. In situ detection of superoxide (middle panel) and hydrogen peroxide (right panel) in leaves was carried out by NBT and DAB staining, respectively. The presence of purple formazan precipitate and brown precipitate indicates the location of superoxide and hydrogen peroxide, respectively. Data is representative of at least three experiments.
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • © The Authors (2009). Journal compilation © New Phytologist (2009)
  • Asset Type:
  • Image
  • Asset Subtype:
  • Image Orientation:
  • Portrait
  • Image Dimensions:
  • 480 x 647
  • Image File Size:
  • 177 KB
  • Creator:
  • Yongli Qiao, Wenzhu Jiang, JooHyun Lee, BongSoo Park, Min‐Seon Choi, Rihua Piao, Mi‐Ok Woo, Jae‐Hwan Roh, Longzhi Han, Nam‐Chon Paek, Hak Soo Seo, Hee‐Jong Koh
  • Credit:
  • Qiao, Y., Jiang, W., Lee, J., Park, B., Choi, M.-S., Piao, R., Woo, M.-O., Roh, J.-H., Han, L., Paek, N.-C., Seo, H. S., & Koh, H.-J. (2010). SPL28 encodes a clathrin‐associated adaptor protein complex 1, medium subunit μ1 (AP1M1) and is responsible for spotted leaf and early senescence in rice (Oryza sativa) New Phytologist, 185(1), 258-274. https://doi.org/10.1111/j.1469-8137.2009.03047.x.
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  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Yongli Qiao, Wenzhu Jiang, JooHyun Lee, BongSoo Park, Min‐Seon Choi, Rihua Piao, Mi‐Ok Woo, Jae‐Hwan Roh, Longzhi Han, Nam‐Chon Paek, Hak Soo Seo, Hee‐Jong Koh
  • Article Copyright Year:
  • 2010
  • Publication Volume:
  • 185
  • Publication Issue:
  • 1
  • Publication Date:
  • 01/01/2010
  • DOI:
  • https://doi.org/10.1111/j.1469-8137.2009.03047.x

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