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  • Description:
  • Gene expression analysis of dbcAMP‐treated retinal pigment epithelium (RPE). (A): Principal component analysis of the microarray gene expression data are shown for samples obtained from three different seeding densities (indicated by the shape of each point: circle, 10,000 cells per cm2; square, 20,000 cells per cm2; diamond, 40,000 cells per cm2) in the presence (blue) or absence (red) of dbcAMP. The data from four time points (day 0, 3, 15, and 34 after seeding) is indicated by the shading of each point and the labeled ellipses such that color intensity increases with increasing time in culture. The day 0 samples are indicated as black points and the proportion of the total variance captured by each principal component is indicated in the axis title. This shows that at each time point tested, there is a “doubling effect,” that is, clustering of samples seeded at half the density in the presence of dbcAMP with samples at double the density but without dbcAMP. (B): Heatmap of gene expression levels for the top 1% most variable genes (rows) observed at day 34 in culture. The seeding density and dbcAMP treatment status each sample is indicated by the labels at the bottom of the heatmap. Expression levels are shown mean centered and scaled to unit variance for each gene. Clustering of samples consistent with the doubling effect can be seen. (C): Heatmap of gene expression levels for selected RPE markers across all timepoints, seeding density and dbcAMP treatment is shown. Abbreviations: BEST1, bestrophin 1; D0, day 0; D3, day 3; D15, day 15; D34, day 34; dbcAMP, dibutyryl‐cAMP; k, ×1,000 cells per cm2; MITF, microphthalmia‐associated transcription factor; RLBP1, retinaldehyde‐binding protein 1; SILV, silver homolog; TYR, tyrosinase.
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • © AlphaMed Press
  • Asset Type:
  • Image
  • Asset Subtype:
  • Figure
  • Image Orientation:
  • Portrait
  • Image Dimensions:
  • x
  • Image File Size:
  • 473 KB
  • Creator:
  • Parul Choudhary, Alex Gutteridge, Emma Impey, R. Ian Storer, Robert M. Owen, Paul J. Whiting, Magda Bictash, Caroline L. Benn
  • Credit:
  • Choudhary, P., Gutteridge, A., Impey, E., Storer, R. I., Owen, R. M., Whiting, P. J., Bictash, M., & Benn, C. L. (2016). Targeting the cAMP and Transforming Growth Factor‐β Pathway Increases Proliferation to Promote Re‐Epithelialization of Human Stem Cell‐Derived Retinal Pigment Epithelium. STEM CELLS Translational Medicine, 5(7), 925-937..
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  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Parul Choudhary, Alex Gutteridge, Emma Impey, R. Ian Storer, Robert M. Owen, Paul J. Whiting, Magda Bictash, Caroline L. Benn
  • Article Copyright Year:
  • 2016
  • Publication Volume:
  • 5
  • Publication Issue:
  • 7
  • Publication Date:
  • 07/01/2016
  • DOI:

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