Asset Details

  • Description:
  • Integration of donor‐derived cells in the epithelial lung compartment. Immunohistological analysis was used to characterize the composition of the epithelial compartment within donor‐derived GFP+ green patches. Imaris software was used to determine fluorescence colocalization. GFP+ areas were designated as the areas of interest, and the colocalization channel identified pixels that included both red and green colors. ATI cells were stained with Aquaporin type 5 (A), ATII cells with surfactant protein C (B), club cells with CCSP (CC16) (C), and CFTR+ cells with anti‐CFTR (D). In each figure, the red channel represents the lineage marker, the green channel marks donor‐derived GFP+ cells, and the blue channel marks nuclear staining. Scale bar = 40 µm. (E): Summary of % ROI (average plus SD) colocalized 8 weeks after transplantation of 8E + 6 adult GFP+ lung cells (based on five different areas obtained from two transplanted mice). Abbreviations: GFP, green fluorescent protein; ROI, region of interest.
  • License:
  • CC-BY-NC-ND
  • Rights Holder:
  • Creative Commons by John Wiley & Sons
  • License Rights Holder:
  • © 2018 AlphaMed Press
  • Asset Type:
  • Image
  • Asset Subtype:
  • Image Orientation:
  • Portrait
  • Image Dimensions:
  • x
  • Image File Size:
  • 620 KB
  • Creator:
  • Irit Milman Krentsis, Chava Rosen, Elias Shezen, Anna Aronovich, Bar Nathanson, Esther Bachar‐Lustig, Neville Berkman, Miri Assayag, Guy Shakhar, Tali Feferman, Ran Orgad, Yair Reisner
  • Credit:
  • Milman Krentsis, I., Rosen, C., Shezen, E., Aronovich, A., Nathanson, B., Bachar‐Lustig, E., Berkman, N., Assayag, M., Shakhar, G., Feferman, T., Orgad, R., & Reisner, Y. (2018). Lung Injury Repair by Transplantation of Adult Lung Cells Following Preconditioning of Recipient Mice. STEM CELLS Translational Medicine, 7(1), 68-77..
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  • Restrictions:
  • This image was published under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, (CC-BY-NC-ND) which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. You may purchase a high resolution image file if your request is non-commercial and you are not adapting the image.
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Irit Milman Krentsis, Chava Rosen, Elias Shezen, Anna Aronovich, Bar Nathanson, Esther Bachar‐Lustig, Neville Berkman, Miri Assayag, Guy Shakhar, Tali Feferman, Ran Orgad, Yair Reisner
  • Article Copyright Year:
  • 2018
  • Publication Volume:
  • 7
  • Publication Issue:
  • 1
  • Publication Date:
  • 01/01/2018
  • DOI:

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