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  • Description:
  • A, BKnockdown of ANO8 (siA8) reduced CRAC current in cells transfected with Orai1 (O1) and STIM1 (S1) and buffered with 3 mM EGTA. CKnockdown of ANO8 reduces the native store‐dependent Ca2+ influx measured in store‐depleted cells by Ca2+ add‐back. D, EKnockdown of ANO8 reduced the number of store‐dependent STIM1 puncta at the TIRF plane in cells expressing STIM1 and Orai1. Panel (D) shows representative images and (E) is the summary of seven experiments. F, GCurrent was measured with pipette solution contacting the fast and strong Ca2+ buffer 10 mM BAPTA in HEK cells transfected with STIM1, Orai1, and with (red) or without ANO8 (black). Panel (G) shows the increase in current density at peak current. Note the prominent current inactivation in the presence of ANO8. HKnockdown of SARAF (red) in wild‐type cells had no effect on current inactivation in the presence of 10 mM BAPTA. IKnockdown of SARAF did not prevent the ANO8‐dependent current inactivation in the presence of 10 mM BAPTA. Data information: The first number in parenthesis indicates the number of similar experiments performed, and the second number is the number of cells analyzed. All results are given as mean ± SEM of the indicated number of experiments or cells analyzed, and differences were analyzed by unpaired t‐test.Source data are available online for this figure.
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • © 2019 EMBO
  • Asset Type:
  • Image
  • Asset Subtype:
  • Chart/Graph
  • Image Orientation:
  • Landscape
  • Image Dimensions:
  • 2128 x 1571
  • Image File Size:
  • 638 KB
  • Creator:
  • Archana Jha, Woo Young Chung, Laura Vachel, Jozsef Maleth, Sarah Lake, Guofeng Zhang, Malini Ahuja, Shmuel Muallem
  • Credit:
  • Jha, A., Chung, W. Y., Vachel, L., Maleth, J., Lake, S., Zhang, G., Ahuja, M., & Muallem, S. (2019). Anoctamin 8 tethers endoplasmic reticulum and plasma membrane for assembly of Ca2+ signaling complexes at the ER/PM compartment. The EMBO Journal, 38(12), n/a..
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  • Keywords:
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  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Archana Jha, Woo Young Chung, Laura Vachel, Jozsef Maleth, Sarah Lake, Guofeng Zhang, Malini Ahuja, Shmuel Muallem
  • Article Copyright Year:
  • 2019
  • Publication Volume:
  • 38
  • Publication Issue:
  • 12
  • Publication Date:
  • 06/17/2019
  • DOI:

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