Asset Details

  • Description:
  • Generation of CAR NK‐92 cells. (a) Lentiviral transfer plasmids encoding full‐length or truncated FLT3‐specific chimeric antigen receptors under the control of the spleen focus forming virus promoter (SFFV). The CAR sequences are followed by an internal ribosome entry site (IRES) and enhanced green fluorescent protein (EGFP) cDNA. In pS‐4G8.28.z‐IEW the CAR consists of an immunoglobulin heavy chain signal peptide (SP), a FLT3‐specific scFv antibody fragment, a Myc‐tag (M), a CD8α hinge region (CD8α), transmembrane and intracellular domains of CD28 and the intracellular domain of CD3ζ (CAR 4G8.28.z), whereas pS‐4G8.TM‐IEW encodes a truncated CAR without signaling capabilities that only harbors the transmembrane domain of CD28 (CAR 4G8.TM). (b) Enhanced green fluorescent protein expression of sorted NK‐92/4G8.28.z and NK‐92/4G8.TM single cell clones was determined by direct flow cytometry (filled areas). (c) CAR surface expression was analyzed by flow cytometry with Myc‐tag‐specific antibody (filled areas). Parental NK‐92 cells served as controls (open areas).
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • © 2019 UICC
  • Asset Type:
  • Image
  • Asset Subtype:
  • Figure
  • Image Orientation:
  • Landscape
  • Image Dimensions:
  • 2128 x 1114
  • Image File Size:
  • 138 KB
  • Creator:
  • Sarah Oelsner, Anja Waldmann, Arne Billmeier, Jasmin Röder, Aline Lindner, Evelyn Ullrich, Rolf Marschalek, Gianpietro Dotti, Gundram Jung, Ludger Große‐Hovest, Pranav Oberoi, Peter Bader, Winfried S. Wels
  • Credit:
  • Oelsner, S., Waldmann, A., Billmeier, A., Röder, J., Lindner, A., Ullrich, E., Marschalek, R., Dotti, G., Jung, G., Große‐Hovest, L., Oberoi, P., Bader, P., & Wels, W. S. (2019). Genetically engineered CAR NK cells display selective cytotoxicity against FLT3‐positive B‐ALL and inhibit in vivo leukemia growth. International Journal of Cancer, 145(7), 1935-1945..
  • Collection:
  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Sarah Oelsner, Anja Waldmann, Arne Billmeier, Jasmin Röder, Aline Lindner, Evelyn Ullrich, Rolf Marschalek, Gianpietro Dotti, Gundram Jung, Ludger Große‐Hovest, Pranav Oberoi, Peter Bader, Winfried S. Wels
  • Article Copyright Year:
  • 2019
  • Publication Volume:
  • 145
  • Publication Issue:
  • 7
  • Publication Date:
  • 10/01/2019
  • DOI:

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