Asset Details

  • Description:
  • Interfacial physico‐chemical properties of LipY domains. (A) Adsorption of LipY, LipYΔPE, LipYΔ149 and LipYΔ170 onto DOPG monomolecular films. Each enzyme (40 nm final concentration) was injected in aqueous phase (10 mm Tris‐HCl buffer (pH 7.5) containing 150 mm NaCl) below the lipid film at various initial surface pressures (Πi). The maximal surface pressure increase (ΔΠmax) was then recorded and plotted as a function of Πi. The critical surface pressure for penetration (Πc; intercept of the linear regression with the x‐axis) and ΔΠ0 (intercept of the linear regression with the y‐axis) were determined. Experiments were carried out in a cylindrical Teflon trough as described in the Materials and Methods section. Data are presented as mean values of two independent assays performed in duplicate (CV% < 5.0%). (B) Temperature dependence of the wavenumber of the asymmetric methylene stretching υas(CH2) for DOPG in the absence and in the presence of LipY, LipYΔPE, LipYΔ149 and LipYΔ170 at protein‐to‐lipid ratio of 1 : 10. Average behaviour of DOPG alone or in the presence of LipYΔPE, LipYΔ149 and LipYΔ170 have been highlighted in thick black and compared to DOPG in the presence of LipY (filled blue dots).
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • Copyright © 2019 Federation of European Biochemical Societies
  • Asset Type:
  • Image
  • Asset Subtype:
  • Figure
  • Image Orientation:
  • Portrait
  • Image Dimensions:
  • 1836 x 2330
  • Image File Size:
  • 441 KB
  • Creator:
  • Pierre Santucci, Nabil Smichi, Sadia Diomandé, Isabelle Poncin, Vanessa Point, Hélène Gaussier, Jean‐François Cavalier, Laurent Kremer, Stéphane Canaan
  • Credit:
  • Santucci, P., Smichi, N., Diomandé, S., Poncin, I., Point, V., Gaussier, H., Cavalier, J.-F., Kremer, L., & Canaan, S. (2019). Dissecting the membrane lipid binding properties and lipase activity of Mycobacterium tuberculosis LipY domains. The FEBS Journal, 286(16), 3164-3181..
  • Collection:
  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Pierre Santucci, Nabil Smichi, Sadia Diomandé, Isabelle Poncin, Vanessa Point, Hélène Gaussier, Jean‐François Cavalier, Laurent Kremer, Stéphane Canaan
  • Article Copyright Year:
  • 2019
  • Publication Volume:
  • 286
  • Publication Issue:
  • 16
  • Publication Date:
  • 08/01/2019
  • DOI:

Click on image to enlarge

Would you like to download this asset? Tell us more and we can generate a quote, license and downloadable file.

Add to Cart