Asset Details

  • Description:
  • A comparison of optical density (OD) measurements at multiple incubation time points and two different concentrations of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) in wells containing Batrachochytrium dendrobatidis (Bd). The MTT assay provides a colorimetric measurement of metabolically active cells. A volume of 20 μL of 5 mg/mL MTT added to 100 μL of Bd culture (purple) produced a greater color change than 10 μL (pink). With the addition of 20 μL 5 mg/mL MTT, the assay was maximized after a 2–4-h incubation (Color figure online).
  • License:
  • Rights Managed
  • Rights Holder:
  • Springer Nature
  • License Rights Holder:
  • © The Author(s) 2019
  • Asset Type:
  • Image
  • Asset Subtype:
  • Chart/Graph
  • Image Orientation:
  • Landscape
  • Image Dimensions:
  • 969 x 824
  • Image File Size:
  • 98.5 KB
  • Creator:
  • Alexa Lindauer, Tiffany May, Gabriela Rios-Sotelo, Ciara Sheets, Jamie Voyles
  • Credit:
  • Lindauer, A., May, T., Rios-Sotelo, G., Sheets, C., & Voyles, J. (2019). Quantifying and Viability. EcoHealth, 16(2), 346-350. https://doi.org/10.1007/s10393-019-01414-6.
  • Collection:
  • Keywords:
  • Batrachochytrium dendrobatidis, Batrachochytrium salamandrivorans, MTT assay, Pathogen, Disease, Amphibian declines
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Alexa Lindauer, Tiffany May, Gabriela Rios-Sotelo, Ciara Sheets, Jamie Voyles
  • Article Copyright Year:
  • 2019
  • Publication Volume:
  • 16
  • Publication Issue:
  • 2
  • Publication Date:
  • 05/23/2019
  • DOI:
  • https://doi.org/10.1007/s10393-019-01414-6

Click on image to enlarge

Would you like to download this asset? Tell us more and we can generate a quote, license and downloadable file.

Add to Cart