Asset Details
- Identifier:
- sct32016591277-fig-0007
- Description:
- Preischemic administration of stromal vascular fraction (SVF) inhibited TGF‐β1‐induced epithelia‐mesenchymal transition (EMT) and microvascular rarefaction. (A–C): Relative abundance of E‐cadherin/GAPDH (A) and α‐SMA/GAPDH (B) was calculated by Western blot assay (C) at 6 months after IR in different groups. (D): Preischemic administration of SVF significantly attenuated the upregulation of α‐SMA/E‐cadherin expression compared with postischemic administration of SVF or PBS (p < .05). (E, F): The expression levels of TGF‐β1 in serum (E) and injured kidney (F) in rats received preischemic administration of SVF were significantly lower than that received postischemic administration of SVF or PBS. (G, H): Mean optical density was evaluated by RECA‐1 staining in glomerular (G) and peritubular (H) areas in kidney sections of rats at 6 months after IR. Preischemic administration of SVF significantly inhibited microvascular rarefaction in kidney sections compared with postischemic administration. (I): Representative microscopic images of RECA‐1 staining preformed on sections of kidneys at 6 months after IR in different groups (×400). Scale bars = 50 μm. ∗, p < .05 (vs. sham, IR+SVF pre‐I, and IR+SVF post‐I); #, p < .05 (vs. IR+SVF post‐I). Abbreviations: GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; IR, ischemia/reperfusion; PBS, phosphate‐buffered saline; RECA‐1, rat endothelial cell antigen‐1; α‐SMA, α‐smooth muscle actin; SVF Post‐I, postischemic administration of stromal vascular fraction; SVF Pre‐I, preischemic administration of stromal vascular fraction; TGF‐β1, transforming growth factor‐β1.
- License:
- Rights Managed
- Rights Holder:
- John Wiley & Sons, Inc.
- License Rights Holder:
- © AlphaMed Press
- Asset Type:
- Image
- Asset Subtype:
- Chart/Graph
- Image Orientation:
- Portrait
- Image Dimensions:
- 788 x 854
- Image File Size:
- 702 KB
- Creator:
- Liuhua Zhou, Luwei Xu, Jiangwei Shen, Qun Song, Ran Wu, Yuzheng Ge, Hui Xin, Jiageng Zhu, Jianping Wu, Ruipeng Jia
- Credit:
- Zhou, L., Xu, L., Shen, J., Song, Q., Wu, R., Ge, Y., Xin, H., Zhu, J., Wu, J., & Jia, R. (2016). Preischemic Administration of Nonexpanded Adipose Stromal Vascular Fraction Attenuates Acute Renal Ischemia/Reperfusion Injury and Fibrosis. STEM CELLS Translational Medicine, 5(9), 1277-1288..
- Collection:
- Keywords:
- Restrictions:
- Property Release:
- No
- Model Release:
- No
- Purchasable:
- Yes
- Sensitive Materials:
- No
- Article Authors:
- Liuhua Zhou, Luwei Xu, Jiangwei Shen, Qun Song, Ran Wu, Yuzheng Ge, Hui Xin, Jiageng Zhu, Jianping Wu, Ruipeng Jia
- Article Copyright Year:
- 2016
- Publication Title:
- STEM CELLS Translational Medicine
- Publication Volume:
- 5
- Publication Issue:
- 9
- Publication Date:
- 09/01/2016
- DOI:
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