Asset Details

  • Description:
  • Models for the resuscitation of VBNC cells. (a) In the absence of AI‐2, LuxPQ acts as a kinase that catalyzes histidine phosphorylation, and phosphate is subsequently transferred to LuxU and LuxO. The combination of LuxU and σ54 promotes sRNAs transcription. Together with chaperone Hfq, it destabilizes the encoding of the transcription factor LuxR, a positive regulator of RpoS. Decreased RpoS activity leads to a decrease in the production of catalase (KatG), which further leads to a sensitivity of cells to peroxide. (b) When the concentration of AI‐2 (yellow triangle) is high, LuxPQ binds autoinducer‐2 and is then converted from kinase to phosphatase. Phosphate is stripped from LuxO and LuxU. Low activity LuxO causes the derepression of LuxR. Then, increased RpoS activity leads to an increase in the production of KatG. Increased antioxidant activity allows VBNC cells to be culturable again. (c) Rpf proteins bind to receptors of Rpf on the VBNC cell's surface, which triggers resuscitation. (d) A complex composed of Rpf and Rif acts on the cell wall. Rpf and Rip are predicted to cleave glycosidic and peptide bonds, respectively. The cell wall is remodeled, which subsequently stimulates the resuscitation of VBNC cells. (e) The Rpf and Rif complex acts on the cell wall of VBNC cells and cleaves it to produce peptidoglycan fragments (purple triangles). The peptidoglycan fragments bind to PknB‐like receptors and trigger resuscitation. G, N‐acetylglucosamine; M, N‐acetylmuramic acid. (f) VBNC cells can resuscitate after the removal of stresses such as oxidation, cold, starvation, osmotic stress and ion stress. (g) The host of VBNC cells may provide nutrition or stimulating factors to promote resuscitation
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • © 2020 Institute of Food Technologists®
  • Asset Type:
  • Image
  • Asset Subtype:
  • Chart/Graph
  • Image Orientation:
  • Landscape
  • Image Dimensions:
  • 1333 x 733
  • Image File Size:
  • 3.28 MB
  • Creator:
  • Kai Dong, Hanxu Pan, Dong Yang, Lei Rao, Liang Zhao, Yongtao Wang, Xiaojun Liao
  • Credit:
  • Dong, K., Pan, H., & Yang, D. (2020). Induction, detection, formation, and resuscitation of viable but non‐culturable state microorganisms. Comprehensive Reviews in Food Science and Food Safety, 19(1), 149-183. https://doi.org/10.1111/1541-4337.12513.
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  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Kai Dong, Hanxu Pan, Dong Yang, Lei Rao, Liang Zhao, Yongtao Wang, Xiaojun Liao
  • Article Copyright Year:
  • 2020
  • Publication Volume:
  • 19
  • Publication Issue:
  • 1
  • Publication Date:
  • 01/01/2020
  • DOI:
  • https://doi.org/10.1111/1541-4337.12513

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