Asset Details

  • Description:
  • Granulocyte differentiation from induced pluripotent stem cells (iPSCs). (A): Schematic three‐step differentiation protocol of mouse corrected CGD‐iPSCs into granulocytes. Step 1: EB formation from day 0 to day 6 in suspension plates. Step 2: EB culture on adherent gelatin‐coated plates in hematopoietic differentiation medium from day 7 to 14–18 days. Cytokines were added at day 7 and after 3 days their concentration was doubled. Step 3: Mature differentiation of cKit+ purified hematopoietic in semi‐solid medium (MethoCult) for additional 14 days (d28‐32). Myeloid colonies were recovered and cytospin preparation were used to perform MGG, IHC‐GP91, NBT, and NET assays. (B): Representative flow cytometry analysis for characterization of early neutrophil progenitors during the adherent culture conditions (step 2, day 18). The gray histogram represents the isotype control while the blue histogram is the fluorescent antibody staining for CD45, cKit, CD11b, CD115, and Ly6G. (C): Representative examples of MGG staining of CGD‐33 clone (left), wt clone (middle), and the corrected cl33‐F8 clone (right). Scale bar: 10 μm. Abbreviations: CGD, chronic granulomatous disease; EB, embryoid body; IHC, immunohistochemistry for GP91 protein; MMG, May–Grunwald Giemsa; NBT, NitroBlue tetrazolium assay; NET, neutrophil extracellular trap; wt, wild‐type.
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • © 2019 AlphaMed Press
  • Asset Type:
  • Image
  • Asset Subtype:
  • Chart/Graph
  • Image Orientation:
  • Portrait
  • Image Dimensions:
  • 2128 x 2229
  • Image File Size:
  • 2.01 MB
  • Creator:
  • Alessandra Castelli, Lucia Susani, Ciro Menale, Sharon Muggeo, Elena Caldana, Dario Strina, Barbara Cassani, Camilla Recordati, Eugenio Scanziani, Francesca Ficara, Anna Villa, Paolo Vezzoni, Marianna Paulis
  • Credit:
  • Castelli, A., Susani, L., Menale, C., Muggeo, S., Caldana, E., Strina, D., Cassani, B., Recordati, C., Scanziani, E., Ficara, F., Villa, A., Vezzoni, P., & Paulis, M. (2019). Chromosome Transplantation: Correction of the Chronic Granulomatous Disease Defect in Mouse Induced Pluripotent Stem Cells. STEM CELLS, 37(7), 876-887..
  • Collection:
  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Alessandra Castelli, Lucia Susani, Ciro Menale, Sharon Muggeo, Elena Caldana, Dario Strina, Barbara Cassani, Camilla Recordati, Eugenio Scanziani, Francesca Ficara, Anna Villa, Paolo Vezzoni, Marianna Paulis
  • Article Copyright Year:
  • 2019
  • Publication Volume:
  • 37
  • Publication Issue:
  • 7
  • Publication Date:
  • 07/01/2019
  • DOI:

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