Asset Details

  • Description:
  • Modified mRNA is superior to nonmodified mRNA in instigating in vivo T cell immunity to RALA complexed mRNA. A–C) Flow cytometric analysis of OT‐I T cell proliferation to immunizations with unmodified or modified OVA mRNA nanocomplexes (A). Histograms showing the proliferation of CFSE labeled OVA‐specific OT‐I T cells in response to intradermal immunization with noncomplexed and RALA OVA mRNA nanocomplexes. (B) Percentages and (C) total number of divided OT‐I T cells in response to intradermal injection of PBS, noncomplexed OVA mRNA, and mRNA OVA nanocomplexes. Means ± SD are shown of a total of three mice per group. *** p < 0.0001 (one‐way ANOVA Newman–Keuls post‐test). D,E) Flow cytometric analysis of antigen‐specific killing of target cells in response to prime and boost vaccination with RALA complexed unmodified OVA mRNA or modified OVA mRNA or complexed in DOTAP/DOPE liposomes. (D) Histogram plots showing the presence of CFSEhi target cells and CFSElow nontarget cells in spleen of immunized mice. (E) Graph showing the percentage of antigen‐specific killing of target cells. Means ± SD are shown of a total of four mice per group; *** p < 0.0001 (one‐way ANOVA Newman–Keuls post‐test).
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • © 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
  • Asset Type:
  • Image
  • Asset Subtype:
  • Illustration
  • Image Orientation:
  • Portrait
  • Image Dimensions:
  • 1331 x 1448
  • Image File Size:
  • 1.62 MB
  • Creator:
  • Vimal K. Udhayakumar, Ans De Beuckelaer, Joanne McCaffrey, Cian M. McCrudden, Jonathan L. Kirschman, Daryll Vanover, Lien Van Hoecke, Kenny Roose, Kim Deswarte, Bruno G. De Geest, Stefan Lienenklaus, Philip J. Santangelo, Johan Grooten, Helen O. McCarthy, Stefaan De Koker
  • Credit:
  • Udhayakumar, V. K., De Beuckelaer, A., McCaffrey, J., McCrudden, C. M., Kirschman, J. L., Vanover, D., Van Hoecke, L., Roose, K., Deswarte, K., De Geest, B. G., Lienenklaus, S., Santangelo, P. J., Grooten, J., McCarthy, H. O., De Koker, S., Sahin, U., Kariko, K., Tureci, O., Van Lint, S., Heirman, C., Thielemans, K., Breckpot, K., Van Lint, S., Renmans, D., Broos, K., Dewitte, H., Lentacker, I., Heirman, C., Breckpot, K., Thielemans, K., Van Lint, S., Thielemans, K., Breckpot, K., Weiner, D. B., Rittig, S. M., Haentschel, M., Weimer, K. J., Heine, A., Muller, M. R., Brugger, W., Horger, M. S., Maksimovic, O., Stenzl, A., Hoerr, I., Rammensee, H. G., Holderried, T. A., Kanz, L., Pascolo, S., Brossart, P., Bol, K. F., Figdor, C. G., Aarntzen, E. H., Welzen, M. E., van Rossum, M. M., Blokx, W. A., van de Rakt, M. W., Scharenborg, N. M., de Boer, A. J., Pots, J. M., Olde Nordkamp, M. A., van Oorschot, T. G., Mus, R. D., Croockewit, S. A., Jacobs, J. F., Schuler, G., Neyns, B., Austyn, J. M., Punt, C. J., Schreibelt, G., de Vries, I. J., Van Lint, S., Goyvaerts, C., Maenhout, S., Goethals, L., Disy, A., Benteyn, D., Pen, J., Bonehill, A., Heirman, C., Breckpot, K., Thielemans, K., Pollard, C., Rejman, J., De Haes, W., Verrier, B., Van Gulck, E., Naessens, T., De Smedt, S., Bogaert, P., Grooten, J., Vanham, G., De Koker, S., Kranz, L. M., Diken, M., Haas, H., Kreiter, S., Loquai, C., Reuter, K. C., Meng, M., Fritz, D., Vascotto, F., Hefesha, H., Grunwitz, C., Vormehr, M., Husemann, Y., Selmi, A., Kuhn, A. N., Buck, J., Derhovanessian, E., Rae, R., Attig, S., Diekmann, J., Jabulowsky, R. A., Heesch, S., Hassel, J., Langguth, P., Grabbe, S., Huber, C., Tureci, O., Sahin, U., Kubler, H., Scheel, B., Gnad‐Vogt, U., Miller, K., Schultze‐Seemann, W., Vom Dorp, F., Parmiani, G., Hampel, C., Wedel, S., Trojan, L., Jocham, D., Maurer, T., Rippin, G., Fotin‐Mleczek, M., von der Mulbe, F., Probst, J., Hoerr, I., Kallen, K. J., Lander, T., Stenzl, A., Fotin‐Mleczek, M., Duchardt, K. M., Lorenz, C., Pfeiffer, R., Ojkic‐Zrna, S., Probst, J., Kallen, K. J., Bettinger, T., Carlisle, R. C., Read, M. L., Ogris, M., Seymour, L. W., Copolovici, D. M., Langel, K., Eriste, E., Langel, U., van Asbeck, A. H., Beyerle, A., McNeill, H., Bovee‐Geurts, P. H., Lindberg, S., Verdurmen, W. P., Hallbrink, M., Langel, U., Heidenreich, O., Brock, R., Deshayes, S., Konate, K., Rydstrom, A., Crombez, L., Godefroy, C., Milhiet, P. E., Thomas, A., Brasseur, R., Aldrian, G., Heitz, F., Munoz‐Morris, M. A., Devoisselle, J. M., Divita, G., McCarthy, H. O., McCaffrey, J., McCrudden, C. M., Zholobenko, A., Ali, A. A., McBride, J. W., Massey, A. S., Pentlavalli, S., Chen, K. H., Cole, G., Loughran, S. P., Dunne, N. J., Donnelly, R. F., Kett, V. L., Robson, T., Bennett, R., Yakkundi, A., McKeen, H. D., McClements, L., McKeogh, T. J., McCrudden, C. M., Arthur, K., Robson, T., McCarthy, H. O., Schach, D. K., Rock, W., Franz, J., Bonn, M., Parekh, S. H., Weidner, T., Anderson, B. R., Muramatsu, H., Nallagatla, S. R., Bevilacqua, P. C., Sansing, L. H., Weissman, D., Kariko, K., Kariko, K., Muramatsu, H., Welsh, F. A., Ludwig, J., Kato, H., Akira, S., Weissman, D., De Beuckelaer, A., Pollard, C., Van Lint, S., Roose, K., Van Hoecke, L., Naessens, T., Udhayakumar, V. K., Smet, M., Sanders, N., Lienenklaus, S., Saelens, X., & Weiss, S., Vanham, G., Grooten, J., De Koker, S., De Beuckelaer, A., Grooten, J., De Koker, S., Lienenklaus, S., Cornitescu, M., Zietara, N., Lyszkiewicz, M., Gekara, N., Jablonska, J., Edenhofer, F., Rajewsky, K., Bruder, D., Hafner, M., Staeheli, P., & Weiss, S. (2017). Arginine‐Rich Peptide‐Based mRNA Nanocomplexes Efficiently Instigate Cytotoxic T Cell Immunity Dependent on the Amphipathic Organization of the Peptide. Advanced Healthcare Materials, 6(13), n/a-n/a..
  • Collection:
  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Vimal K. Udhayakumar, Ans De Beuckelaer, Joanne McCaffrey, Cian M. McCrudden, Jonathan L. Kirschman, Daryll Vanover, Lien Van Hoecke, Kenny Roose, Kim Deswarte, Bruno G. De Geest, Stefan Lienenklaus, Philip J. Santangelo, Johan Grooten, Helen O. McCarthy, Stefaan De Koker
  • Article Copyright Year:
  • 2017
  • Publication Volume:
  • 6
  • Publication Issue:
  • 13
  • Publication Date:
  • 07/01/2017
  • DOI:

Click on image to enlarge

Would you like to download this asset? Tell us more and we can generate a quote, license and downloadable file.

Add to Cart