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  • Description:
  • The interaction of bba-milR1 with the target gene CLIPB9. a Diagram of the mosquito melanization response. Clip domain serine proteinase: CLIPB9; Serine protease inhibitor: SRPN2; prophenoloxidase: PPO; phenoloxidase: PO. b The relative transcript levels of CLIPB9 in mosquitoes at 36 h post infection with WT, milR1-OV 1#, or milR1-KO 1# strains. There was no significant difference in CLIPB9 expression among the groups at this time point (Student’s t test). c The relative transcript levels of CLIPB9 in mosquitoes at 84 h post infection with WT, milR1-OV 1#, or milR1-KO 1# strains. The expression values are normalized to WT. d Infection of milR1-OV1# and milR1-KO 1# results in significant increase and decrease in A. stephensi hemolymph PO activity compared with WT at 84 h post infection, respectively. e Effect of CLIPB9 silencing on fungal hyphal body formation in the mosquito hemocoel at 60 hpi. The black arrows and red arrow indicate the hyphal bodies and mosquito hemocyte, respectively. Scale bars, 10 μm. f qPCR-based quantification of fungal load in mosquitoes injected with dsGFP or dsCLIPB9 at 60 hpi. Fungal levels are expressed as that of fungal 18S rRNA relative to A. stephensi ribosomal protein S7 (AsS7) DNA. g Effect of CLIPB9 silencing on the survival of mosquitoes following topical application of a suspension of 107 conidia/ml of B. bassiana ARSEF252 (Log-rank test). Values are mean ± s.e.m. Similar results were obtained in three biological repeats. *P < 0.05, **P < 0.01, ***P < 0.001. P-value < 0.05 was regarded as statistically significant (Student’s t test). Source data are provided as a Source Data file
  • License:
  • CC-BY
  • Rights Holder:
  • Springer Nature
  • License Rights Holder:
  • © The Author(s) 2019
  • Asset Type:
  • Image
  • Asset Subtype:
  • Figure
  • Image Orientation:
  • Portrait
  • Image Dimensions:
  • 997 x 1977
  • Image File Size:
  • 467 KB
  • Creator:
  • Chunlai Cui, Yan Wang, Jingnan Liu, Jing Zhao, Peilu Sun, Sibao Wang
  • Credit:
  • Cui, C., Wang, Y., Liu, J., Zhao, J., Sun, P., & Wang, S. (2019). A fungal pathogen deploys a small silencing RNA that attenuates mosquito immunity and facilitates infection. Nature Communications, 10(1), 4298. https://doi.org/10.1038/s41467-019-12323-1.
  • Collection:
  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Chunlai Cui, Yan Wang, Jingnan Liu, Jing Zhao, Peilu Sun, Sibao Wang
  • Article Copyright Year:
  • 2019
  • Publication Volume:
  • 10
  • Publication Issue:
  • 1
  • Publication Date:
  • 09/20/2019
  • DOI:
  • https://doi.org/10.1038/s41467-019-12323-1

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