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  • Description:
  • NTN4 Increases EPC Migration, Proliferation, and Angiogenic Activity through a UNC5B‐dependent Mechanism. A, NTN4 mRNA levels and those of related receptors were determined by RT‐PCR in S/LEPCs and HUVECs. Glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) was used as an internal control. B, SEPCs were transfected with control siRNA (siCtrl) or UNC5B‐specific siRNA (siUNC5B), and mRNA and protein levels of UNC5B were assessed by RT‐PCR and western blot. Error bars indicate ± SEM (n = 5). *P < .05 and **P < .01 vs siCtrl. Student's t test was applied. C,D, The NTN4 regulates SEPCs proliferation (n = 3) and motility (n = 6) through UNC5B. The silencing of UNC5B reduced NTN4‐mediated SEPC proliferation (C) and motility (D). Error bars indicate ± SEM. **P < .01 vs siCtrl. Student's t test was applied. E,F, NTN4‐primed siCtrl and siUNC5B SEPCs were co‐culture with HUVECs in the transwell chamber. Representative images of scratch‐wound healing (E) and capillary like tube formation (F) by HUVECs. Error bars indicate ± SEM (n = 4). **P < .01 vs siCtrl. Student's t test was applied. Scale bar, 50 µm (E) and 100 µm (F), respectively
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • © 2020 Federation of American Societies for Experimental Biology
  • Asset Type:
  • Image
  • Asset Subtype:
  • Figure
  • Image Orientation:
  • Landscape
  • Image Dimensions:
  • 2084 x 938
  • Image File Size:
  • 550 KB
  • Creator:
  • Na Geum Lee, In Cheul Jeung, Soon Chul Heo, Jinhoi Song, Wooil Kim, Byungtae Hwang, Min‐Gi Kwon, Yeon‐Gu Kim, Jangwook Lee, Jong‐Gil Park, Min‐Gyeong Shin, Young‐Lai Cho, Mi‐Young Son, Kwang‐Hee Bae, Sang‐Hyun Lee, Jae Ho Kim, Jeong‐Ki Min
  • Credit:
  • Lee, N., Jeung, I., & Heo, S. (2020). Ischemia‐induced Netrin‐4 promotes neovascularization through endothelial progenitor cell activation via Unc‐5 Netrin receptor B. The FASEB Journal, 34(1), 1231-1246. https://doi.org/10.1096/fj.201900866RR.
  • Collection:
  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Na Geum Lee, In Cheul Jeung, Soon Chul Heo, Jinhoi Song, Wooil Kim, Byungtae Hwang, Min‐Gi Kwon, Yeon‐Gu Kim, Jangwook Lee, Jong‐Gil Park, Min‐Gyeong Shin, Young‐Lai Cho, Mi‐Young Son, Kwang‐Hee Bae, Sang‐Hyun Lee, Jae Ho Kim, Jeong‐Ki Min
  • Article Copyright Year:
  • 2020
  • Publication Volume:
  • 34
  • Publication Issue:
  • 1
  • Publication Date:
  • 01/01/2020
  • DOI:
  • https://doi.org/10.1096/fj.201900866RR

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