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  • Description:
  • EGCs prevent neuronal cell death and early apoptosis induced by dopamine in primary cultures of ENS. A–D) Primary cultures of ENS were treated without dopamine (control; A, C) orwithdopamine (B, D) for 24 h in the presence (C, D)or absence (A, B) of EGCs and stained with antibodies against β‐tubulin III. Images are representative of 1 of 6 independentexperiments. E) Quantification of NSE released in the culture medium of primary cultures of ENS not treated (open bars) or treated with dopamine (solid bars) in the presence or absence of EGCs (+ EGC and −EGC, respectively). Values are means ± se (n independent experiments). F–K) Images of primary cultures of ENS treated without dopamine (control; F, G) or with dopamine (H–K)for6hintheabsence (H, I) or presence (J, K) of EGCs seeded in transwell filters. Primary cultures were stained with antibodies against Hu (F, H, J) and active caspase‐3 (G, I, K). Arrows indicate colocalization of active caspase‐3 with Hu. L) Quantitative analysis of the proportion of active caspase‐3 IR neurons (caspase 3‐IR) vs. total neurons identified with Hu (20 ganglia/condition). Values are means ± se (nindependent experiments). *P< 0.05 vs. control; #P< 0.05 vs. other treatment; 1‐way ANOVA with Tukey's post hoc test. Scale bars = 100 μm(C); 50 μm(F, G).
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • © Federation of American Societies for Experimental Biology
  • Asset Type:
  • Image
  • Asset Subtype:
  • Figure
  • Image Orientation:
  • Landscape
  • Image Dimensions:
  • 1800 x 1084
  • Image File Size:
  • 852 KB
  • Creator:
  • Hind Abdo, Pascal Derkinderen, Priya Gomes, Julien Chevalier, Philippe Aubert, Damien Masson, Jean-Paul Galmiche, Pieter Vanden Berghe, Michel Neunlist, Bernard Lardeux
  • Credit:
  • Abdo, H., Derkinderen, P., Gomes, P., Chevalier, J., Aubert, P., Masson, D., Galmiche, J.-P., Berghe, P. V., Neunlist, M., & Lardeux, B. (2010). Enteric glial cells protect neurons from oxidative stress in part via reduced glutathione. The FASEB Journal, 24(4), 1082-1094..
  • Collection:
  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Hind Abdo, Pascal Derkinderen, Priya Gomes, Julien Chevalier, Philippe Aubert, Damien Masson, Jean-Paul Galmiche, Pieter Vanden Berghe, Michel Neunlist, Bernard Lardeux
  • Article Copyright Year:
  • 2010
  • Publication Volume:
  • 24
  • Publication Issue:
  • 4
  • Publication Date:
  • 04/01/2010
  • DOI:

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