Asset Details

  • Description:
  • Characterization of reprogrammed cells from D‐MEFs. A) Morphological analysis of reprogrammed clones. a–c) I‐3 (a), II‐25 (b), and III‐10 (c) are representative clones from classes I, II, and III, respectively. Top panel: initial appearance of colonies. Middle panel: passaged culture of each clone. Bottom panel: AP staining. d, e) MEFs (d) and HM1 mouse ESCs (e, top panel) and their AP staining (e, bottom panel) are shown as controls. Initial colonies were imaged using an ×40 objective, the rest with an ×10 objective. Scale bars = 100 µm. B, C) Expression levels of pluripotency markers (B) and retroviral transgenes (C) in representative clones of each class of reprogrammed cells, as determined by RT‐PCR. Nat1 was used as an internal control. NTC, no template control.
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • © Federation of American Societies for Experimental Biology
  • Asset Type:
  • Image
  • Asset Subtype:
  • Figure
  • Image Orientation:
  • Landscape
  • Image Dimensions:
  • 1800 x 1107
  • Image File Size:
  • 851 KB
  • Creator:
  • Renjith Matthew, Wenwen Jia, Arati Sharma, Yuanjun Zhao, Loren E. Clarke, Xiang Cheng, Huayan Wang, Ugur Salli, Kent E. Vrana, Gavin P. Robertson, Jiyue Zhu, Shuwen Wang
  • Credit:
  • Mathew, R., Jia, W., Sharma, A., Zhao, Y., Clarke, L. E., Cheng, X., Wang, H., Salli, U., Vrana, K. E., Robertson, G. P., Zhu, J., & Wang, S. (2010). Robust activation of the human but not mouse telomerase gene during the induction of pluripotency. The FASEB Journal, 24(8), 2702-2715. www.fasebj.org.
  • Collection:
  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Renjith Matthew, Wenwen Jia, Arati Sharma, Yuanjun Zhao, Loren E. Clarke, Xiang Cheng, Huayan Wang, Ugur Salli, Kent E. Vrana, Gavin P. Robertson, Jiyue Zhu, Shuwen Wang
  • Article Copyright Year:
  • 2010
  • Publication Volume:
  • 24
  • Publication Issue:
  • 8
  • Publication Date:
  • 08/01/2010
  • DOI:
  • www.fasebj.org

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