Asset Details

  • Description:
  • Location of the proteolytic activity. (A) Scheme of the separation of the egg‐jelly components by means of their differential solubility in 70% ethanol, presence (+) and absence (–) of proteolytic activity. (B) Effect of the time of incubation on SNBP degradation. SNBP (200 µg protein) were incubated with whole egg‐jelly (0.4 µg protein/µL) or with the insoluble macromolecular fraction (0.4 µg protein/µL) of the egg‐jelly (ethyl alcohol (EtOH) pp). Both egg‐jelly and EtOH pp fractions were reconstituted by dialysis in buffer A. The reactions were carried out at 40 µL final volume. Reactions were stopped by adding 40 µL of acetic‐urea PAGE sample buffer (2x). The percentage of degradation was determined with respect to the control for each incubation time. (C) Effect of the concentration of the whole egg‐jelly and EtOH pp on SNBP degradation. The conditions used were the same as in (B) and the incubation time was 15 min.
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • Asset Type:
  • Image
  • Asset Subtype:
  • Chart/Graph
  • Image Orientation:
  • Portrait
  • Image Dimensions:
  • x
  • Image File Size:
  • 43.5 KB
  • Creator:
  • Luis J. Del Valle
  • Credit:
  • Del Valle, L. J. (2005). In vitro decondensation of the sperm chromatin in Holothuria tubulosa (sea cucumber) not affecting proteolysis of basic nuclear proteins. Development, Growth & Differentiation, 47(5), 333-342. https://doi.org/10.1111/j.1440-169X.2005.00805.x.
  • Collection:
  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Luis J. Del Valle
  • Article Copyright Year:
  • 2005
  • Publication Volume:
  • 47
  • Publication Issue:
  • 5
  • Publication Date:
  • 06/01/2005
  • DOI:
  • https://doi.org/10.1111/j.1440-169X.2005.00805.x

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