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/assets?dynamic_filters%5Barticle_title%5D=An+Effective+Method+for+Quantifying+RNA+Expression+of+IbsC%E2%80%90SibC%2C+a+Type+I+Toxin%E2%80%90Antitoxin+System+in+Escherichia+coli&template=grid

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- cbic202000280-fig-0004
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- John Wiley & Sons, Inc.
Description Growth‐phase dependency of ibsC toxin and sibC antitoxin gene expressions. Our optimized RT‐qPCR‐based approach was used as previously described to analyse gene expression. The results were...
Article Title: An Effective Method for Quantifying RNA Expression of IbsC‐SibC, a Type I Toxin‐Antitoxin System in Escherichia coli
Publication Title: ChemBioChem

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- Identifier
- cbic202000280-fig-0005
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- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description Average cellular IbsC and SibC RNA copy numbers in E. coli cells at mid‐log phase (OD600≈0.5) in balanced growth at 37 °C in LB media. RT‐qPCR was used to measure target RNA copy numbers in total...
Article Title: An Effective Method for Quantifying RNA Expression of IbsC‐SibC, a Type I Toxin‐Antitoxin System in Escherichia coli
Publication Title: ChemBioChem

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- Identifier
- cbic202000280-fig-0007
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- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description Growth impairment vs. measured IbsC toxin and SibC antitoxin RNA levels in the engineered E. coli cells under various Atc and IPTG conditions. A) Growth curves for DH5αZ1 (diamonds), DH5αZ1PE...
Article Title: An Effective Method for Quantifying RNA Expression of IbsC‐SibC, a Type I Toxin‐Antitoxin System in Escherichia coli
Publication Title: ChemBioChem

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- Identifier
- cbic202000280-fig-0002
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- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description Next‐generation sequencing with standard IbsC and SibC DNA samples. A) Workflow and list of samples tested. Each sample contained known copy numbers of SibC or IbsC DNA obtained by OD260. B)...
Article Title: An Effective Method for Quantifying RNA Expression of IbsC‐SibC, a Type I Toxin‐Antitoxin System in Escherichia coli
Publication Title: ChemBioChem

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- Identifier
- cbic202000280
- License Type
- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description An Effective Method for Quantifying RNA Expression of IbsC‐SibC, a Type I Toxin‐Antitoxin System in Escherichia coli - ChemBioChemChemBioChem vol. 21, November 2, 2020
Article Title: An Effective Method for Quantifying RNA Expression of IbsC‐SibC, a Type I Toxin‐Antitoxin System in Escherichia coli
Publication Title: ChemBioChem

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- Identifier
- cbic202000280-fig-0001
- License Type
- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description Establishing an effective RT‐qPCR method for IbsC and SibC RNA detection. A) Overall workflow used to obtain the required materials for RT‐qPCR. B) Amplification curves. NRT: no reverse...
Article Title: An Effective Method for Quantifying RNA Expression of IbsC‐SibC, a Type I Toxin‐Antitoxin System in Escherichia coli
Publication Title: ChemBioChem

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- Identifier
- cbic202000280-fig-0003
- License Type
- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description Next‐generation sequencing to confirm specificity of IbsC and SibC RNA detection and to validate quantification by RT‐qPCR. A) Work flow to generate the next‐generation sequencing library. Standard...
Article Title: An Effective Method for Quantifying RNA Expression of IbsC‐SibC, a Type I Toxin‐Antitoxin System in Escherichia coli
Publication Title: ChemBioChem

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