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Description Characterization of the corrected cl33‐F8 clone. (A): Left: histogram showing the chromosome distribution at passages 13 (p13) and 20 (p20). Right: representative DAPI‐banding karyotype. The...
Article Title: Chromosome Transplantation: Correction of the Chronic Granulomatous Disease Defect in Mouse Induced Pluripotent Stem Cells
Publication Title: STEM CELLS -
Description Analysis of the chromosome‐transplanted HATR cl33‐F clone. (A): Histogram showing the chromosome distribution (left) and representative DAPI‐banding karyotypes at passage 3 (p3) of the two 41,XXY...
Article Title: Chromosome Transplantation: Correction of the Chronic Granulomatous Disease Defect in Mouse Induced Pluripotent Stem Cells
Publication Title: STEM CELLS -
Description Granulocyte differentiation from induced pluripotent stem cells (iPSCs). (A): Schematic three‐step differentiation protocol of mouse corrected CGD‐iPSCs into granulocytes. Step 1: EB formation from...
Article Title: Chromosome Transplantation: Correction of the Chronic Granulomatous Disease Defect in Mouse Induced Pluripotent Stem Cells
Publication Title: STEM CELLS -
Description Schematic overview of the “chromosome therapy” approach in CGD‐iPSCs. The scheme shows the step‐by‐step procedure followed to generate and correct mouse CGD‐iPSCs in which an endogenous chromosome...
Article Title: Chromosome Transplantation: Correction of the Chronic Granulomatous Disease Defect in Mouse Induced Pluripotent Stem Cells
Publication Title: STEM CELLS -
Description Analysis of differentiated cells from the defective CGD‐33 clone (left), wt clone (middle), and the corrected cl33‐F8 clone (right). (A): IHC with an antibody to GP91. The cells were counterstained...
Article Title: Chromosome Transplantation: Correction of the Chronic Granulomatous Disease Defect in Mouse Induced Pluripotent Stem Cells
Publication Title: STEM CELLS -
Description Hprt inactivation of the CGD‐33 clone. (A): Schematic representation of the cotransfection strategy: CGD‐induced pluripotent stem cells were cotransfected with a CRISPR/Cas9 plasmid containing a...
Article Title: Chromosome Transplantation: Correction of the Chronic Granulomatous Disease Defect in Mouse Induced Pluripotent Stem Cells
Publication Title: STEM CELLS