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Description Peak‐picking and second derivative spectrum for selecting intense IR bands. Left: peak‐picking on specific absorption spectrum for determining the first series of IR‐bands on curve‐fitting; Right:...
Article Title: Quantitative IR microscopy and spectromics open the way to 3D digital pathology
Publication Title: Journal of Biophotonics -
Description 3D visible image of mouse brain tissue blocks with anatomical features. A = brain section diagram with main tissue areas: the blue rectangle represents the tissue area analyzed in B and C; the red...
Article Title: Quantitative IR microscopy and spectromics open the way to 3D digital pathology
Publication Title: Journal of Biophotonics -
Description 3D IR image reconstruction for spectral‐derived features of tissue sub‐structures. Top‐left: 2D IR image of single wavelength absorption (1424 cm–1) revealing blood vessels in a tissue section of...
Article Title: Quantitative IR microscopy and spectromics open the way to 3D digital pathology
Publication Title: Journal of Biophotonics -
Description Iterative curve‐fitting of the 3050–900 cm–1 spectral interval. Left: spectral curve‐fitting using the position of the 67 bands defined on 2nd step and by limiting the free parameters of band shape...
Article Title: Quantitative IR microscopy and spectromics open the way to 3D digital pathology
Publication Title: Journal of Biophotonics -
Description 3D IR image of FAC unsaturation level in mouse brain tissue. Top‐left: 2D IR image of lipids (∫(3020–2800 cm–1) for a tissue section of mouse brain at interaural 3.45 mm/bregma –0.34 mm (central...
Article Title: Quantitative IR microscopy and spectromics open the way to 3D digital pathology
Publication Title: Journal of Biophotonics -
Description Baseline correction and removal of generic bands in tissue FTIR spectra. Left = correction of baseline (raw spectrum and baseline‐corrected spectrum are shown); Right = generic absorptions from the...
Article Title: Quantitative IR microscopy and spectromics open the way to 3D digital pathology
Publication Title: Journal of Biophotonics