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Description Setup for dual delivery of morphogen‐containing media. (A) A large polystyrene box is used to hold two 50‐ml conical tubes. The figure shows example drill locations for connecting tubing to...
Article Title: Spatiotemporal Control of Morphogen Delivery to Pattern Stem Cell Differentiation in Three‐Dimensional Hydrogels
Publication Title: Current Protocols in Stem Cell Biology -
Description Calculating, modeling, and validating diffusion coefficients. (A‐C) By tracking diffusion profiles and fitting the resultant data to a semi‐infinite slab model as described in the text, a diffusion...
Article Title: Spatiotemporal Control of Morphogen Delivery to Pattern Stem Cell Differentiation in Three‐Dimensional Hydrogels
Publication Title: Current Protocols in Stem Cell Biology -
Description Patterns of MSC differentiation can be tuned based on morphogen concentration. In these experiments, an MSC‐laden hydrogel was perfused for 35 days with osteogenic and chondrogenic media from...
Article Title: Spatiotemporal Control of Morphogen Delivery to Pattern Stem Cell Differentiation in Three‐Dimensional Hydrogels
Publication Title: Current Protocols in Stem Cell Biology -
Description Troubleshooting Guidelines
Article Title: Spatiotemporal Control of Morphogen Delivery to Pattern Stem Cell Differentiation in Three‐Dimensional Hydrogels
Publication Title: Current Protocols in Stem Cell Biology -
Description Assembly of cell‐laden hydrogels within the bioreactor. To generate the cell‐laden hydrogel, a 10% gelatin mixture and 20% microbial transglutaminase (mTG) mixture are separately mixed and...
Article Title: Spatiotemporal Control of Morphogen Delivery to Pattern Stem Cell Differentiation in Three‐Dimensional Hydrogels
Publication Title: Current Protocols in Stem Cell Biology -
Description Imaging morphogen diffusion using confocal microscopy. (A‐B) In this example, two fluorescently labeled dextran molecules of the same size were perfused through opposite channels. Fluorescence...
Article Title: Spatiotemporal Control of Morphogen Delivery to Pattern Stem Cell Differentiation in Three‐Dimensional Hydrogels
Publication Title: Current Protocols in Stem Cell Biology -
Description Bioreactor fabrication and assembly process. A 3D‐printed jig is used to guide drilling of holes for the perfusion ports (A‐B), which are then fitted with Luer bulkheads and locking nuts. One‐inch...
Article Title: Spatiotemporal Control of Morphogen Delivery to Pattern Stem Cell Differentiation in Three‐Dimensional Hydrogels
Publication Title: Current Protocols in Stem Cell Biology -
Description Media reservoir fabrication and assembly process. First, a 3D‐printed jig is used to guide drilling of the holes for the perfusion ports (A). The holes are then marked and drilled (B‐C). Once...
Article Title: Spatiotemporal Control of Morphogen Delivery to Pattern Stem Cell Differentiation in Three‐Dimensional Hydrogels
Publication Title: Current Protocols in Stem Cell Biology -
Description Example of live‐cell imaging of cellular responsive to morphogens using fluorescent reporters. As described in the main text, this series of experiments utilized constitutive RFP‐expressing...
Article Title: Spatiotemporal Control of Morphogen Delivery to Pattern Stem Cell Differentiation in Three‐Dimensional Hydrogels
Publication Title: Current Protocols in Stem Cell Biology -
Description Representative examples of MSC differentiation to osteogenic and chondrogenic fates after long‐term culture in perfused hydrogels. After 35 days of perfusion of osteogenic and chondrogenic media...
Article Title: Spatiotemporal Control of Morphogen Delivery to Pattern Stem Cell Differentiation in Three‐Dimensional Hydrogels
Publication Title: Current Protocols in Stem Cell Biology -
Description Example of MSC fate patterning by delivery of soluble morphogens from distinct locations. In these experiments, an MSC‐laden hydrogel was perfused for 35 days with osteogenic and chondrogenic media...
Article Title: Spatiotemporal Control of Morphogen Delivery to Pattern Stem Cell Differentiation in Three‐Dimensional Hydrogels
Publication Title: Current Protocols in Stem Cell Biology