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Description Effect of the overexpression of POG1 on yeast cell growth. Saccharomyces cerevisiae BY4741 cells harboring pAD4 (WT) and pAD4‐POG1 (+POG1) were grown to the exponential growth phase (OD600 nm of...
Article Title: The transcriptional activator Pog1 controls cell cycle, and its phosphorylated form is downregulated by the ubiquitin ligase Dma2 in Saccharomyces cerevisiae
Publication Title: FEMS Yeast Research -
Description Phosphorylation status of Pog1 during cell cycle. Saccharomyces cerevisiae BY4741 cells harboring pAD4‐POG1 (overexpression of POG1) were synchronized at the G1 phase by α‐factor and released from...
Article Title: The transcriptional activator Pog1 controls cell cycle, and its phosphorylated form is downregulated by the ubiquitin ligase Dma2 in Saccharomyces cerevisiae
Publication Title: FEMS Yeast Research -
Description Oligonucleotide primers used in this study
Article Title: The transcriptional activator Pog1 controls cell cycle, and its phosphorylated form is downregulated by the ubiquitin ligase Dma2 in Saccharomyces cerevisiae
Publication Title: FEMS Yeast Research -
Description Prediction for phosphorylation site in Pog1
Article Title: The transcriptional activator Pog1 controls cell cycle, and its phosphorylated form is downregulated by the ubiquitin ligase Dma2 in Saccharomyces cerevisiae
Publication Title: FEMS Yeast Research -
Description Western blot analysis of Pog1. (a) Saccharomyces cerevisiae BY4741 cells harboring YEp24‐HADMA2, pMyc‐Ubi/323, and pAD4 (WT) or YEp24‐HADMA2, pMyc‐Ubi/323, and pAD4‐POG1 (+POG1) or (b) S....
Article Title: The transcriptional activator Pog1 controls cell cycle, and its phosphorylated form is downregulated by the ubiquitin ligase Dma2 in Saccharomyces cerevisiae
Publication Title: FEMS Yeast Research -
Description Protein interaction between Pog1 and Dma2. Saccharomyces cerevisiae trp1‐disrupted strain (Δtrp1) was used as the host for two‐hybrid analysis. Cells of each strain were incubated on SC‐ura‐his‐trp...
Article Title: The transcriptional activator Pog1 controls cell cycle, and its phosphorylated form is downregulated by the ubiquitin ligase Dma2 in Saccharomyces cerevisiae
Publication Title: FEMS Yeast Research -
Description Genes isolated in yeast two‐hybrid screening
Article Title: The transcriptional activator Pog1 controls cell cycle, and its phosphorylated form is downregulated by the ubiquitin ligase Dma2 in Saccharomyces cerevisiae
Publication Title: FEMS Yeast Research -
Description Phosphorylation of Ser152 and Thr253 of Pog1. (a) Saccharomyces cerevisiae BY4741 cells expressing pAD4‐derived wild‐type Pog1 (WT) and Ala‐substituted Pog1 mutants (S152A, T253A, and S152A/T253A)...
Article Title: The transcriptional activator Pog1 controls cell cycle, and its phosphorylated form is downregulated by the ubiquitin ligase Dma2 in Saccharomyces cerevisiae
Publication Title: FEMS Yeast Research