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Description Quality control of hPSC hypothalamic differentiations by immunocytochemistry. (A) Immunofluorescent staining on Day 10 of hES cells differentiated to dorsal forebrain (cortical) cells. The vast...
Article Title: Generation and Characterization of Functional Human Hypothalamic Neurons
Publication Title: Current Protocols in Neuroscience -
Description Strain mean correlations among variables. Diagonal: Correlations shown in boxes on the diagonal are between the current data, collected in the Windsor laboratory of Douglas Wahlsten as a part of...
Article Title: Overview of Mouse Assays of Ethanol Intoxication
Publication Title: Current Protocols in Neuroscience -
Description Trait correlation table. Values of IAL were compared to all 5104 traits in the BXD Published Phenotypes database. The top 500 correlations ranked by the Genetic Correlation (Pearson's r) are...
Article Title: Systems Genetic Analysis in GeneNetwork.org
Publication Title: Current Protocols in Neuroscience -
Description Cre‐On/Off transgene expression in DIO, DO, and FAS rAAVs. (A) Oppositely oriented loxP (orange triangles) and lox2272 (green triangles) sites permit Cre‐mediated recombination and inversion of the...
Article Title: Cre Activated and Inactivated Recombinant Adeno‐Associated Viral Vectors for Neuronal Anatomical Tracing or Activity Manipulation
Publication Title: Current Protocols in Neuroscience -
Description The main pathways for molecular traffic across the BBB. The paracellular pathway is regulated by the tight junctions that restrict the movement of water‐soluble agents (A); small lipophilic agents...
Article Title: Primary Porcine Brain Microvessel Endothelial Cell Isolation and Culture
Publication Title: Current Protocols in Neuroscience -
Description Initiation and propagation of membrane lipid peroxidation.
Article Title: Measurement of Oxygen Radicals and Lipid Peroxidation in Neural Tissues
Publication Title: Current Protocols in Neuroscience -
Description Stereotaxic injection of lentivirus into mouse brain. Cartoon depicting preparation for intracranial virus injection into the mouse forebrain using a 1‐µl Hamilton syringe; stereotactic apparatus...
Article Title: Lentiviral Strategies for RNAi Knockdown of Neuronal Genes
Publication Title: Current Protocols in Neuroscience -
Description Combinatorial viral delivery to study pathway‐specific gene function. (A) Combined viral injections of a conditional AAV‐FLEX construct (red) into a region containing a neuronal population of...
Article Title: Manipulating Gene Expression in Projection‐Specific Neuronal Populations Using Combinatorial Viral Approaches
Publication Title: Current Protocols in Neuroscience -
Description Lowering background by washing with SDS and sodium deoxycholate (DOC). In this experiment, BW5147 cells (mouse thymoma) labeled with [35S]methionine for 1 hr were extracted with nondenaturing lysis...
Article Title: Immunoprecipitation
Publication Title: Current Protocols in Neuroscience -
Description Troubleshooting Guide
Article Title: Chronic Intravenous Drug Self‐Administration in Rats and Mice
Publication Title: Current Protocols in Neuroscience -
Description Setting the visibility (red circle) of annotations to specific magnification levels.
Article Title: How to Prepare Neuroanatomical Image Data
Publication Title: Current Protocols in Neuroscience -
Description The CB1 antagonist rimonabant (10 mg/kg, i.p.) fully prevents the effects of THC (10 mg/kg, i.p.) on tetrad phenotypes in C57Bl/6J mice. (A) Rimonabant (Rimo) administration fully blocks the...
Article Title: Cannabinoid‐Induced Tetrad in Mice
Publication Title: Current Protocols in Neuroscience -
Description Anesthetized mice should be shaved in the areas marked by cross‐hatching. These areas drain to the axillary and inguinal lymph nodes.
Article Title: Experimental Autoimmune Encephalomyelitis (EAE)
Publication Title: Current Protocols in Neuroscience -
Description Immunoblotting with a semidry transfer unit. Generally, the lower electrode is the anode, and one gel is transferred at a time. A Mylar mask (optional in some units) is put in place on the anode....
Article Title: Immunoblotting and Immunodetection
Publication Title: Current Protocols in Neuroscience -
Description Arrangement of the Gene Gun and sample for ballistic delivery of dye‐coated particles. (A) The outer guard of the barrel liner supplied by Bio‐Rad is cut off to facilitate insertion of the membrane...
Article Title: Ballistic Labeling with Fluorescent Dyes and Indicators
Publication Title: Current Protocols in Neuroscience -
Description Identification of D1 receptor–interacting proteins using immunoprecipitation and mass spectroscopy. (A) Coomassie‐blue stained gel of immunoprecipitated proteins. The D1‐FLAG lane shows proteins...
Article Title: Identifying Novel Protein‐Protein Interactions Using Co‐Immunoprecipitation and Mass Spectroscopy
Publication Title: Current Protocols in Neuroscience -
Description Olfactory habituation/dishabituation setup as described in . For a slant‐top cage lid (left), insert the applicator through the water bottle hole. For a flat‐top cage lid (right), insert the...
Article Title: Simple Behavioral Assessment of Mouse Olfaction
Publication Title: Current Protocols in Neuroscience -
Description Acquisition of schedule‐induced polydipsia by Sprague‐Dawley rats. Food pellets were delivered under a fixed‐time 60 sec (FT‐60) schedule for a duration of 2 hr. Experimental sessions were...
Article Title: Schedule‐Induced Polydipsia: A Rat Model of Obsessive‐Compulsive Disorder
Publication Title: Current Protocols in Neuroscience -
Description Tyrosine phosphorylation of NMDA receptor subunits. Soluble extracts from synaptosomal membrane fraction of rat striatum were precipitated with anti‐phosphotyrosine antibody. Different amounts of...
Article Title: Biochemical Fractionation of Brain Tissue for Studies of Receptor Distribution and Trafficking
Publication Title: Current Protocols in Neuroscience -
Description Chromatograms of Glu (A) and GABA (B) in mouse nucleus accumbens dialysis samples. The system used is described in . A 1‐mm probe was used and the dialysis flow rate was 0.7 µl/min. A volume of 4...
Article Title: Detection and Quantification of Neurotransmitters in Dialysates
Publication Title: Current Protocols in Neuroscience -
Description Double RNA in situ hybridization in E12.5 and E15.5 telencephalon. (A,A',B,B') Coexpression of Bhlhe22 (purple) and Neurog2 (orange). (C,C',D,D') Coexpression of Neurod1 (purple) and Neurog2...
Article Title: Non‐isotopic RNA In Situ Hybridization on Embryonic Sections
Publication Title: Current Protocols in Neuroscience -
Description Schematic of a typical ELISA configuration for measurement of Aβ(1‐42) in guanidine extracts of human brain tissue. The capture antibody is electrostatically adsorbed to the ELISA plate. The...
Article Title: Methods for Sample Preparation for Direct Immunoassay Measurement of Analytes in Tissue Homogenates: ELISA Assay of Amyloid β‐Peptides
Publication Title: Current Protocols in Neuroscience -
Description Spectral sensitivity for CCDs in three different configurations, along with the performance of the human eye for both scotopic and photopic vision.
Article Title: Electronic Imaging in Neuroscience
Publication Title: Current Protocols in Neuroscience -
Description Current traces in response to −5 mV square‐wave voltage step in (A) cell‐attached mode with electrode capacitance compensated. (B) Whole‐cell mode. Scale bars show 50 msec and 50 pA.
Article Title: Electrophysiological Recordings from Neonatal Neocortical Brain Slices
Publication Title: Current Protocols in Neuroscience -
Description (A) Changes over days in body weight relative to weight on day 0 until the first Active rat was removed from the experiment (see Basic Protocol, step 17 for additional details about the 25% removal...
Article Title: Rodent Model of Activity‐Based Anorexia
Publication Title: Current Protocols in Neuroscience -
Description (A) Standard curve and (B) final dose‐response curve for carbachol‐stimulated cAMP accumulation in CHO cells stably expressing the M1 muscarinic receptor.
Article Title: Scintillation Proximity Assay
Publication Title: Current Protocols in Neuroscience -
Description Representative learning curve for escape rats. Mean (±SEM) latency in seconds to terminate the tail shock stress in the wheel‐turn box for escape rats during an 80‐trial session (each block...
Article Title: Rodent Models of Depression: Learned Helplessness Using a Triadic Design in Rats
Publication Title: Current Protocols in Neuroscience -
Description Mean (±SEM) session latency to cross for rats exposed to inescapable shock or restraint and administered either saline, chlordiazepoxide, buspirone, or gepirone (n = 10 rats/group). Asterisk...
Article Title: Rodent Models of Depression: Learned Helplessness Using a Triadic Design in Rats
Publication Title: Current Protocols in Neuroscience -
Description Schematic of template preparation for antisense and sense probes. (A) Generation of antisense DNA template. Restriction enzyme 1 (RE1) specifically recognizes and cleaves the plasmid at the 5′ end...
Article Title: Non‐isotopic RNA In Situ Hybridization on Embryonic Sections
Publication Title: Current Protocols in Neuroscience -
Description pCGF (C‐terminal GFP Fusion) vectors for use in the yeast S. cerevisiae. The galactose‐inducible GAL1 promoter (Yocum et al., ) is used to drive expression of a GFP fusion protein. A protein may be...
Article Title: Use of the A. Victoria Green Fluorescent Protein to Study Protein Dynamics In Vivo
Publication Title: Current Protocols in Neuroscience