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Description Primer Sequences Used for Real‐Time qPCR
Article Title: A High‐Throughput Screening Method to Identify Compounds Displaying Human Vascular Embryonic Toxicity
Publication Title: Current Protocols in Stem Cell Biology -
Description Troubleshooting Guide
Article Title: A High‐Throughput Screening Method to Identify Compounds Displaying Human Vascular Embryonic Toxicity
Publication Title: Current Protocols in Stem Cell Biology -
Description Comparison of CTB differentiation from StemPro‐ versus E8‐adapted WA09/H9 hESCs, using BMP4+IWP2. (A) First‐step differentiation of the StemPro‐ and E8‐adapted H9s do not show any morphological...
Article Title: An Improved Two‐Step Protocol for Trophoblast Differentiation of Human Pluripotent Stem Cells
Publication Title: Current Protocols in Stem Cell Biology -
Description List of Primary Antibodies
Article Title: Differentiation of Retinal Organoids from Human Pluripotent Stem Cells
Publication Title: Current Protocols in Stem Cell Biology -
Description Immunoblot of p21 and actin expressions in cells from TIC‐low and TIC‐high culture. Lysates of TIC‐high and TIC‐low cultured cells were loaded after determining the protein concentration. Lysates...
Article Title: In Vitro Enrichment of Tumor‐Initiating Cells from Human Established Cell Lines
Publication Title: Current Protocols in Stem Cell Biology -
Description List of Reagents Used in the Protocols
Article Title: Differentiation of Retinal Organoids from Human Pluripotent Stem Cells
Publication Title: Current Protocols in Stem Cell Biology -
Description Representative flow cytometry plots of phenotypic evaluation of TIC‐low and TIC‐high fractions obtained from the various tumor cell lines. Cells from TIC‐high and TIC‐low cultures of all cell lines...
Article Title: In Vitro Enrichment of Tumor‐Initiating Cells from Human Established Cell Lines
Publication Title: Current Protocols in Stem Cell Biology -
Description Analysis of drug toxicity under flow conditions. (A) Schematic representation of the experiments performed to evaluate the effect of the model drug 7‐cyclo in hPSC‐derived ECs cultured under flow...
Article Title: A High‐Throughput Screening Method to Identify Compounds Displaying Human Vascular Embryonic Toxicity
Publication Title: Current Protocols in Stem Cell Biology -
Description Immunohistological analysis in 3D and 2D cultures. (A) Stereomicroscopic (SM) image of BMP/SB‐treated aggregates at day 7. (B) Magnification of boxed region in (A). The small vesicle is encircled...
Article Title: Generation of Functional CX26–Gap‐Junction‐Plaque‐Forming Cells with Spontaneous Ca2+ Transients via a Gap Junction Characteristic of Developing Cochlea
Publication Title: Current Protocols in Stem Cell Biology -
Description Schematic diagram of two‐step trophoblast differentiation protocol using human pluripotent stem cells (hPSCs). Basic Protocol 1 describes the expansion of hPSCs to prepare the starting material for...
Article Title: An Improved Two‐Step Protocol for Trophoblast Differentiation of Human Pluripotent Stem Cells
Publication Title: Current Protocols in Stem Cell Biology -
Description Tumor tissue in vivo xenograft. (A) Human melanoma tissues. (B) Melanoma tissues were cut into small pieces. (C) Cut a small incision on mouse skin. (D) Outstretch a subcutaneous pocket. (E) Insert...
Article Title: Isolation of Human Melanoma Stem Cells Using ALDH as a Marker
Publication Title: Current Protocols in Stem Cell Biology -
Description Immunohistochemistry for CX26 and CX30 in mouse cochlea and differentiated cells from 2D culture. (A) CX26 (red) expression at apical end of P6 mouse cochlea. Nuclei were stained with DAPI (blue)....
Article Title: Generation of Functional CX26–Gap‐Junction‐Plaque‐Forming Cells with Spontaneous Ca2+ Transients via a Gap Junction Characteristic of Developing Cochlea
Publication Title: Current Protocols in Stem Cell Biology -
Description Representative flow cytometry plots for the analysis of ALDH enzymatic activity in cells from TIC‐low and TIC‐high cultures. Cells from TIC‐high and TIC‐low cultures were tested for ALDH enzymatic...
Article Title: In Vitro Enrichment of Tumor‐Initiating Cells from Human Established Cell Lines
Publication Title: Current Protocols in Stem Cell Biology -
Description Subrenal capsule injection. (A) The mouse is positioned with its left side up. A left flank incision is made just under the costal margin. (B) The kidney is gently pulled out of the abdominal...
Article Title: Colon Cancer Stem Cells
Publication Title: Current Protocols in Stem Cell Biology -
Description Representative images of immunohistochemical characterization of developing retinal organoids. (A) Retinal organoids contain a thick layer of retinal progenitor cells (VSX2, green), some...
Article Title: Differentiation of Retinal Organoids from Human Pluripotent Stem Cells
Publication Title: Current Protocols in Stem Cell Biology -
Description Immunohistochemistry for CX26 and SOX2 in mouse cochlea and differentiated cells from 2D culture. (A‐C) Staining for CX26 (green), SOX2 (red), and DAPI (blue) in 16‐week mouse cochlea....
Article Title: Generation of Functional CX26–Gap‐Junction‐Plaque‐Forming Cells with Spontaneous Ca2+ Transients via a Gap Junction Characteristic of Developing Cochlea
Publication Title: Current Protocols in Stem Cell Biology -
Description Schematic demonstrating two different approaches to achieving a tightly controlled, intermediate level of signaling. (A) Top‐down inhibition (TDi) saturates cells within the culture with a positive...
Article Title: Top‐Down Inhibition (TDi) and Baseline Activation (BLa): Controlling Signal Transduction When Endogenous Cytokines are Ruining Your Differentiation
Publication Title: Current Protocols in Stem Cell Biology -
Description Melanoma‐initiating cells forming floating sphere‐like structures in vitro. Tumor spheres of a patient‐derived metastatic melanoma (A, B) and of a commercial melanoma cell line (A375) (C, D), using...
Article Title: Culture and Isolation of Melanoma‐Initiating Cells
Publication Title: Current Protocols in Stem Cell Biology -
Description Immunohistochemistry to detect CX26 and p27kip1 in mouse cochlea and differentiated cells from 2D culture. (A and B) Staining for CX26 (red), p27kip1 (green), and DAPI (blue) in P6 mouse cochlea....
Article Title: Generation of Functional CX26–Gap‐Junction‐Plaque‐Forming Cells with Spontaneous Ca2+ Transients via a Gap Junction Characteristic of Developing Cochlea
Publication Title: Current Protocols in Stem Cell Biology -
Description Representative image of HEMA 3–stained myotubes before (A) and after (B) selecting myotubes and example results diagram (C). Arrows in A represent a subset of myotubes that will be selected. Arrow...
Article Title: HEMA 3 Staining: A Simple Alternative for the Assessment of Myoblast Differentiation
Publication Title: Current Protocols in Stem Cell Biology -
Description Setup for dual delivery of morphogen‐containing media. (A) A large polystyrene box is used to hold two 50‐ml conical tubes. The figure shows example drill locations for connecting tubing to...
Article Title: Spatiotemporal Control of Morphogen Delivery to Pattern Stem Cell Differentiation in Three‐Dimensional Hydrogels
Publication Title: Current Protocols in Stem Cell Biology -
Description Comparison of trophoblast differentiation using BMP4, with and without IWP2, starting with E8‐adapted WA09/H9 hESCs. (A) Morphology of the cells in IWP2 shows more uniform flattening starting from...
Article Title: An Improved Two‐Step Protocol for Trophoblast Differentiation of Human Pluripotent Stem Cells
Publication Title: Current Protocols in Stem Cell Biology -
Description Functional analysis of iPS cell–derived CX26‐GJP‐forming cells. (A) Dye transfer using Lucifer yellow (LY; green). (B) Immunostaining for CX26 (red) in same region shown in (A). (C) Magnification...
Article Title: Generation of Functional CX26–Gap‐Junction‐Plaque‐Forming Cells with Spontaneous Ca2+ Transients via a Gap Junction Characteristic of Developing Cochlea
Publication Title: Current Protocols in Stem Cell Biology -
Description Representative two‐step differentiation data from StemFlex‐adapted WA09/H9 hESCs. (A) Morphology of the cells during the first step of differentiation (day 0 to day 4). Cells are plated (day 1) in...
Article Title: An Improved Two‐Step Protocol for Trophoblast Differentiation of Human Pluripotent Stem Cells
Publication Title: Current Protocols in Stem Cell Biology -
Description Generation of PDTX model. Clinical melanoma tissues were defined as F0. Tumors were serially passaged into nude mice as F1, F2,…Fn.
Article Title: Isolation of Human Melanoma Stem Cells Using ALDH as a Marker
Publication Title: Current Protocols in Stem Cell Biology -
Description Calculating, modeling, and validating diffusion coefficients. (A‐C) By tracking diffusion profiles and fitting the resultant data to a semi‐infinite slab model as described in the text, a diffusion...
Article Title: Spatiotemporal Control of Morphogen Delivery to Pattern Stem Cell Differentiation in Three‐Dimensional Hydrogels
Publication Title: Current Protocols in Stem Cell Biology -
Description Sample results.
Article Title: Culture and Isolation of Brain Tumor Initiating Cells
Publication Title: Current Protocols in Stem Cell Biology -
Description Patterns of MSC differentiation can be tuned based on morphogen concentration. In these experiments, an MSC‐laden hydrogel was perfused for 35 days with osteogenic and chondrogenic media from...
Article Title: Spatiotemporal Control of Morphogen Delivery to Pattern Stem Cell Differentiation in Three‐Dimensional Hydrogels
Publication Title: Current Protocols in Stem Cell Biology -
Description Minimum Information to be Recorded and Reported for a Flow Cytometry Experiment
Article Title: Reliable Protocols for Flow Cytometry Analysis of Intracellular Proteins in Pluripotent Stem Cell Derivatives: A Fit‐For‐Purpose Approach
Publication Title: Current Protocols in Stem Cell Biology -
Description (A) Six explants from a GBM cultured on a Millicell insert placed on a 35‐mm culture dish. (B) Heterogenic tissues from a single GBM tumor on a 100‐mm culture dish. Upper row from the left: gray...
Article Title: Organotypic Explant Culture of Glioblastoma Multiforme and Subsequent Single‐Cell Suspension
Publication Title: Current Protocols in Stem Cell Biology