Advanced Search
Displaying assets from 1 to 30 out of 1337
-
Description The cartridge/hollow fiber culturing system. (A) Priming and normal function; (B) loading of endothelial cells and glia; (C) testing permeability.
Article Title: Development of an In Vitro Blood‐Brain Barrier
Publication Title: Current Protocols in Toxicology -
Description Decay factors for calculating the amount of radioactivity present at a given time after a reference date. For example, a vial containing 1.85 MBq (50 µCi) of an 35S‐labeled compound on the...
Article Title: Safe Use of Radioisotopes
Publication Title: Current Protocols in Toxicology -
Description Example of data sheet used for the observations in .
Article Title: Methods in the Analysis of Maternal Behavior in the Rodent
Publication Title: Current Protocols in Toxicology -
Description Reported SHE Cell Morphological Transformation Frequencies for Select Chemicals
Article Title: Cell Transformation Assays
Publication Title: Current Protocols in Toxicology -
Description Recommended plate map for initial assay optimization.
Article Title: Fluorescent pH and Oxygen Probes of the Assessment of Mitochondrial Toxicity in Isolated Mitochondria and Whole Cells
Publication Title: Current Protocols in Toxicology -
Description Historical Control Data on Selected Reproductive Parameters in CD‐1 (Swiss) Mice
Article Title: Guidelines for Mating Rodents
Publication Title: Current Protocols in Toxicology -
Description Extinction Coefficients of Reagents
Article Title: Immuno‐Spin Trapping: Detection of Protein‐Centered Radicals
Publication Title: Current Protocols in Toxicology -
Description Outline of the procedures used for the extraction, purification, derivatization, and mass spectrometric analysis of F2‐IsoPs from biological sources.
Article Title: Quantification of F2‐Isoprostanes by Gas Chromatography/Mass Spectrometry as a Measure of Oxidant Stress
Publication Title: Current Protocols in Toxicology -
Description After 4 days of treatment, tamoxifen did not cause multinucleation at any of the concentrations tested. A cytotoxic concentration of 20 µM resulted in about 80% viability after 2 days, whereas no...
Article Title: Assessing Cell Fusion and Cytokinesis Failure as Mechanisms of Clone 9 Hepatocyte Multinucleation In Vitro
Publication Title: Current Protocols in Toxicology -
Description Representative results of HO‐1 measurements using the HTRF assay. The positive control (50 µm CdCl2) and three kidney toxic compounds (cisplatin, citrinin, and arsenic trioxide) show...
Article Title: A High‐Throughput Screening Assay to Identify Kidney Toxic Compounds
Publication Title: Current Protocols in Toxicology -
Description MT detection by immunoblot. Protein samples were heated in the water bath for 20 min prior to being run on a 15% SDS/acrylamide gel. The gel was first run at 22 mA until the bromphenol blue entered...
Article Title: Detection and Manipulation of the Stress Response Protein Metallothionein
Publication Title: Current Protocols in Toxicology -
Description MPT monitored with a Δψ‐sensitive electrode. Mitochondria (1 mg/ml) were incubated in KCl‐based buffer. After a 1‐min stabilization period mitochondria were loaded with Ca2+, and, when accumulation...
Article Title: Analysis of Mitochondrial Dysfunction During Cell Death
Publication Title: Current Protocols in Toxicology -
Description Flux (%dose/hr) versus time profiles for carbaryl in acetone (circles) or DMSO (squares) vehicles in a flow‐through diffusion cell system.
Article Title: Porcine Skin Flow‐Through Diffusion Cell System
Publication Title: Current Protocols in Toxicology -
Description Preparation of Dilutions for the Assay
Article Title: Analysis of Immunotoxicity by Enumeration of Antibody‐Producing B Cells
Publication Title: Current Protocols in Toxicology -
Description PCR Solutions for Genotyping GCL Transgenic Mice
Article Title: Glutamate Cysteine Ligase (GCL) Transgenic and Gene‐Targeted Mice for Controlling Glutathione Synthesis
Publication Title: Current Protocols in Toxicology -
Description Plot in the analyzed mode showing percentage of DNA damage.
Article Title: Inter‐and Intra‐Laboratory Standardization of TUNEL Assay for Assessment of Sperm DNA Fragmentation
Publication Title: Current Protocols in Toxicology -
Description Human Hepatic NAT Activities
Article Title: Measuring the Activity of Arylamine N‐Acetyltransferase (NAT)
Publication Title: Current Protocols in Toxicology -
Description Chromatogram from HPLC determination of cysteinesulfinate production. Asparagine (Asn) is an internal standard. No cysteic acid is apparent in this sample. The sample being chromatographed is from...
Article Title: Measurement of Cysteine Dioxygenase Activity and Protein Abundance
Publication Title: Current Protocols in Toxicology -
Description Schematic of dorsal view of mouse lung lobes. Lobes are named as described by Cook et al. (). The pattern of lobation may vary with the strain of mouse.
Article Title: Murine Asthma Models
Publication Title: Current Protocols in Toxicology -
Description Sample experimental plate setup. In one 96‐well plate, it is possible to run 4 drugs with 8 time points (t0, t1, t2…t5, t6, t7) in triplicate. At each time‐point, 50 µl of supernatant from each...
Article Title: Micropatterned Co‐Cultures of Human Hepatocytes and Stromal Cells for the Assessment of Drug Clearance and Drug‐Drug Interactions
Publication Title: Current Protocols in Toxicology -
Description pI Values of Some Human Aldehyde Dehydrogenases
Article Title: Aldehyde Dehydrogenases: Measurement of Activities and Protein Levels
Publication Title: Current Protocols in Toxicology -
Description SLC assay for optimizing construct pairs using dengue virus type 2 (DENV2) NS2B‐NS3 interaction. (A) Experimental design using 96‐well plate. (B) Relative luminescence units (RLU) generated by each...
Article Title: Analysis of Protein‐Protein Interactions by Split Luciferase Complementation Assay
Publication Title: Current Protocols in Toxicology -
Description Protein‐to‐Protein Variation
Article Title: The Colorimetric Detection and Quantitation of Total Protein
Publication Title: Current Protocols in Toxicology -
Description Coronal sections of the brain of 14‐day‐old ischemic rat pups (7 days after the hypoxic‐ischemic procedure) stained with toluidine blue (on the left), and right and left areas and volumes...
Article Title: Experimental Models of Hypoxic‐Ischemic Encephalopathy: Hypoxia‐Ischemia in the Immature Rat
Publication Title: Current Protocols in Toxicology -
Description Timing by Experimental Task
Article Title: Synaptic Structure Quantification in Cultured Neurons
Publication Title: Current Protocols in Toxicology -
Description Dose‐response curve for a specific ChE inhibitor. Iso‐OMPA inhibits human plasma BChE, but not RBC AChE, at 10−4 M. This inhibitor concentration can be used to distinguish between the two enzymes...
Article Title: Determination of Cholinesterase in Blood and Tissue
Publication Title: Current Protocols in Toxicology -
Description Fluorescence‐based capillary sequence analysis of MDR1 wild type and an MDR1 knockout clone analyzed using ABI Peak Scanner software. (A) The wild‐type sequence is 263.01 bp. (B) Three knockout...
Article Title: Use of Zinc Finger Nuclease Technology to Knock Out Efflux Transporters in C2BBe1 Cells
Publication Title: Current Protocols in Toxicology -
Description Abbreviations, Chemical Names, Molecular Weights, GC Retention Times, and Prominent m/z Peaks Observed in the GC‐MS Electron Impact Spectra of Cathinones
Article Title: Gas Chromatography Mass Spectrometry (GC‐MS) for Identification of Designer Stimulants Including 2C Amines, NBOMe Compounds, and Cathinones in Urine
Publication Title: Current Protocols in Toxicology -
Description Pb suppressed CMP differentiation into myeloid cells in mice. B6 mice were treated with 1250 ppm of Pb via drinking water for 8 weeks. Thereafter, CMP (gated on Lin‐c‐KithiScal‐1‐CD34+CD16/32‐) in...
Article Title: Phenotypic and Functional Evaluation of Hematopoietic Stem and Progenitor Cells in Toxicology of Heavy Metals
Publication Title: Current Protocols in Toxicology -
Description Representative substrates and hydrolytic reactions catalyzed by human CEs. (A) Narcotics (cocaine and heroin), (B) chemotherapeutic agent (CPT‐11), (C) standard substrates used in...
Article Title: Enzymatic Activity of Human Carboxylesterases
Publication Title: Current Protocols in Toxicology