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Description Composition and interchange of Hsp90‐based chaperone complexes. Schematic diagram showing the composition of, and transition between the 'intermediate' and 'mature' Hsp90 chaperone complexes,...
Article Title: Regulation of Hsp90 ATPase activity by tetratricopeptide repeat (TPR)‐domain co‐chaperones
Publication Title: The EMBO Journal -
- Identifier
- embj201591739-fig-0004ev
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Immunofluorescence analysis shows intestinal lesions with accumulation of β‐catenin and SOX9 in the small intestine and the colon, 25 days after induction. Scale bar, 50 μm.
Article Title: E‐cadherin can limit the transforming properties of activating β‐catenin mutations
Publication Title: The EMBO Journal -
Description Processing of OPA1 after dissipation of membrane potential. (A) HeLa cells stained by MitoTracker were cultured in the presence of 20 μM CCCP for 0.25 or 1 h. After 1 h culture, CCCP was washed out...
Article Title: Regulation of mitochondrial morphology through proteolytic cleavage of OPA1
Publication Title: The EMBO Journal -
Description pH dependence of PEG‐assisted [35S]DRM conversion reactions. Conversion reactions with microsomes and [35S]DRMs and 30% PEG treatment were performed as in Figure 6. Following PEG treatment, the...
Article Title: Conversion of raft associated prion protein to the protease‐resistant state requires insertion of PrP‐res (PrPSc) into contiguous membranes
Publication Title: The EMBO Journal -
Description The recombination enhancer mapped by primer extension analysis of micrococcal nuclease‐hypersensitive sites. (A) Chromatin structure of the Crick strand around the first α2 repressor‐binding site...
Article Title: Cell type‐specific chromatin organization of the region that governs directionality of yeast mating type switching
Publication Title: The EMBO Journal -
Description SsrA RNA co‐sediments with 70S ribosomes in lysates of wild‐type but not ΔsmpB‐1 cells. (A) W3110 and W3110 ΔsmpB‐1 cell lysates were fractionated using the centrifugation and washing steps shown...
Article Title: SmpB, a unique RNA‐binding protein essential for the peptide‐tagging activity of SsrA (tmRNA)
Publication Title: The EMBO Journal -
Description IEF analysis of shCD1b lipid binding. (A) SDS–PAGE gel (12%) of purified shCD1b expressed in mouse cells (first lane) or in insect cells (third lane). Note that shCD1b heavy chains are...
Article Title: Endogenous phosphatidylcholine and a long spacer ligand stabilize the lipid‐binding groove of CD1b
Publication Title: The EMBO Journal -
- Identifier
- embj201695957-fig-0003ev
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description HeLa cells expressing SOD1(A4V)‐GFP were heat‐stressed for 2 h and fixed. G3BP1 was then detected by immunofluorescence, and poly(A) RNA was detected by fluorescence in situ hybridization. In the...
Article Title: An aberrant phase transition of stress granules triggered by misfolded protein and prevented by chaperone function
Publication Title: The EMBO Journal -
- Identifier
- embj2008206-fig-0005
- License Type
- CC-BY-NC-ND
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Receptors containing the β1‐integrin subunit are major candidates for mediating LM1‐induced cell biomimetic elasticity in MECs. (A and B) Elastic moduli of SCp2 cells cultured on top of Matrigel in...
Article Title: Laminin and biomimetic extracellular elasticity enhance functional differentiation in mammary epithelia
Publication Title: The EMBO Journal -
Description Model for Rqh1 controlling recombination at stalled replication forks. In this version of the model, the replication fork is blocked by a UV photoproduct (solid triangle) in the leading strand...
Article Title: Partial suppression of the fission yeast rqh1− phenotype by expression of a bacterial Holliday junction resolvase
Publication Title: The EMBO Journal -
- Identifier
- embj7601830-fig-0003
- License Type
- CC-BY-NC-ND
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Analysis of mouse Sho in tissue preparations. (A) Expression of Sho in whole mouse embryos assessed by Western blotting using a 12% Tris–glycine gel. Sho is expressed beginning at embryonic day 16....
Article Title: The CNS glycoprotein Shadoo has PrPC‐like protective properties and displays reduced levels in prion infections
Publication Title: The EMBO Journal -
Description In vivo growth characteristics of VEGF−/− fibrosarcoma cell lines. (A) Three independent clones of H‐ras‐transformed VEGF−/− cells (528ras1, ‐3 and ‐5) form aggressive tumors in SCID mice upon s.c....
Article Title: Contrasting effects of VEGF gene disruption in embryonic stem cell‐derived versus oncogene‐induced tumors
Publication Title: The EMBO Journal -
Description Position 32 of several tRNAs and position 34 of tRNAPhe and tRNATrp are not 2′‐O‐methylated in a trm7Δ strain. Autoradiograms of selected two‐dimensional TLC of modified mono‐ and dinucleotides...
Article Title: Trm7p catalyses the formation of two 2′‐O‐methylriboses in yeast tRNA anticodon loop
Publication Title: The EMBO Journal -
Description Model for the functions of cohesin in the DNA damage response. DNA damage sensors (ATM, ATR, and the MRE11–RAD50–NBS1 complex) are activated in G1, S, and/or G2–M phase. In S, phosphorylated NBS1...
Article Title: Cohesin's dual role in the DNA damage response: repair and checkpoint activation
Publication Title: The EMBO Journal -
- Identifier
- embj2019101863-fig-0008
- License Type
- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description A–CHeLa cells were transfected with siRNA and subjected to immunofluorescence staining with DAPI, ACA, and the anti‐PICH antibody. Example images of anaphase cells are shown (A). The percentage of...
Article Title: Histone H2A phosphorylation recruits topoisomerase IIα to centromeres to safeguard genomic stability
Publication Title: The EMBO Journal -
Description Near‐UV difference CD spectra for Hsp90–co‐chaperone interactions. Near‐UV difference CD spectra for titration of co‐chaperones into Hsp90, obtained by subtracting the spectra of the free...
Article Title: Regulation of Hsp90 ATPase activity by tetratricopeptide repeat (TPR)‐domain co‐chaperones
Publication Title: The EMBO Journal -
- Identifier
- embj201796525-fig-0003
- License Type
- CC-BY-NC-ND
- Rights Holder
- Creative Commons by John Wiley & Sons
Description AThermodynamic characterization of the RBPJ/L3MBTL3 interaction. Representative thermograms (raw heat signal and nonlinear least squares fit to the integrated data) for L3MBTL3‐(31‐70) binding to...
Article Title: RBPJ/CBF1 interacts with L3MBTL3/MBT1 to promote repression of Notch signaling via histone demethylase KDM1A/LSD1
Publication Title: The EMBO Journal -
- Identifier
- embj201695553-fig-0002
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description BJ human fibroblasts (proliferating, G; and DNA damage‐induced senescent, DIS) were transduced with either siRNA targeting p21 (sip21) or control siRNA (siCtrl). Cells were harvested and analyzed...
Article Title: p21 maintains senescent cell viability under persistent DNA damage response by restraining JNK and caspase signaling
Publication Title: The EMBO Journal -
Description Creation of SOD2‐overexpressing mice. (A) Contig of the PAC clones used to generate transgenic mice. (B) Southern blot analysis of tail DNA from transgenic founder animals probed with human SOD2...
Article Title: SOD2 overexpression: enhanced mitochondrial tolerance but absence of effect on UCP activity
Publication Title: The EMBO Journal -
Description GATA‐binding activities in larval fat body extracts. (A) EMSA with nuclear extract from third instar larval fat body (lanes 1–3) and a 32P‐labelled GATA probe (wt). Unlabelled competitors were...
Article Title: Serpent regulates Drosophila immunity genes in the larval fat body through an essential GATA motif
Publication Title: The EMBO Journal -
Description CLF protein is nuclear localised but is not present in nuclei throughout mitosis. (A) Root of a clf‐50/clf‐50 35S∷GFP‐CLF plant showing GFP expression in most cells in the nuclei. (B) Close‐up of...
Article Title: Silencing by plant Polycomb‐group genes requires dispersed trimethylation of histone H3 at lysine 27
Publication Title: The EMBO Journal -
Description Polypurine tracts (PPTs) and possible U‐boxes of various retroviruses and retroviral elements
Article Title: Identification of a sequence element immediately upstream of the polypurine tract that is essential for replication of simian immunodeficiency virus
Publication Title: The EMBO Journal -
Description (A–C) Immunoisolation of proOmpA‐BPTI with translocase. Translocation reactions (1.5 ml) were performed as described above using IMVs with similar amounts of SecE and SecEHA in the presence or...
Article Title: Evaluating the oligomeric state of SecYEG in preprotein translocase
Publication Title: The EMBO Journal -
Description ATF‐2 functions as a repressor of IL‐8 transcription. (A) HeLa cells were transfected with empty or ATF‐2‐expressing vector and 40 h following transfection the cells were infected with Sendai virus...
Article Title: Epigenetic determination of a cell‐specific gene expression program by ATF‐2 and the histone variant macroH2A
Publication Title: The EMBO Journal -
Description mCRY1 physically interacts with the C‐terminus of mPER2 and promotes nuclear accumulation rather than translocation of full‐length mPER2. (A) Identification of the mCRY1 binding region in mPER2....
Article Title: Nucleocytoplasmic shuttling and mCRY‐dependent inhibition of ubiquitylation of the mPER2 clock protein
Publication Title: The EMBO Journal -
- Identifier
- embj201694105-fig-0005
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description AEM analysis of the yNap1c2–H2AΔ14–H2BΔ28 complex. Representative raw image of negatively stained particles. BRepresentative particles (1st row), class averages coming from the projection matching...
Article Title: Structural evidence for Nap1‐dependent H2A–H2B deposition and nucleosome assembly
Publication Title: The EMBO Journal -
Description Transcriptional pulse–chase analysis of the PGK1pG reporter. Wild‐type and dhh1Δ cells carrying the GAL:PGK1pG reporter (Decker and Parker, 1993) were grown to an OD600 of 0.6. Transcription of the...
Article Title: The DEAD box protein Dhh1 stimulates the decapping enzyme Dcp1
Publication Title: The EMBO Journal -
- Identifier
- embj201696247-fig-0002
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Representative images (left) and quantifications (right) of the number of intestinal progenitor cells in control midguts or midguts in which Ret has been downregulated from adult ISCs/EBs [achieved...
Article Title: Ret receptor tyrosine kinase sustains proliferation and tissue maturation in intestinal epithelia
Publication Title: The EMBO Journal -
Description MBD1‐SUMO1 interacts with CAF‐1 but not with SETDB1. (A) MBD1 but not MBD1‐SUMO1 co‐immunoprecipitates with SETDB1 from nuclear extracts of cells expressing GFP‐PIAS1 and GFP‐PIAS3. (B) MBD1 and...
Article Title: Regulation of MBD1‐mediated transcriptional repression by SUMO and PIAS proteins
Publication Title: The EMBO Journal -
Description Binding site mapping. (A) The upper portion presents far‐western blots (see Materials and methods) showing the relative ability of GST fusions with clathrin fragments to associate with the D4...
Article Title: Crystal structure of a 12 ANK repeat stack from human ankyrinR
Publication Title: The EMBO Journal