Advanced Search
Displaying assets from 1 to 30 out of 18617
-
Description Mice were injected via the tail vein with Ad‐GFP or Ad‐shRNA for LSD1, and 2 weeks later, mice were fasted for 12 h and then treated with FGF19 for 2 h (A, C, E) or refed for 6 h (B, D, F). A,...
Article Title: A postprandial FGF19‐SHP‐LSD1 regulatory axis mediates epigenetic repression of hepatic autophagy
Publication Title: The EMBO Journal -
- Identifier
- embj2018100809-fig-0006
- License Type
- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description AExample trace of PP7‐GAL10 transcription in 2% galactose. BBoxplot of the onset of the first transcription event in different doses of galactose. The box indicates quartiles, the horizontal line...
Article Title: Live‐cell imaging reveals the interplay between transcription factors, nucleosomes, and bursting
Publication Title: The EMBO Journal -
Description PKAr is predicted to possess two cAMP‐binding sites. A single glycine to glutamic acid change in the site B of the cAMP‐binding domain is known to abolish cAMP binding and to prevent activation of...
Article Title: Crosstalk between PKA and PKG controls pH‐dependent host cell egress of Toxoplasma gondii
Publication Title: The EMBO Journal -
Description The crystal structure of the BH3‐in‐groove Bax homodimer (PDB entry 4BDU) is shown with one monomer colored gray and the other colored green, magenta, blue, and red for its α2, α3, α4, and α5...
Article Title: BH3‐in‐groove dimerization initiates and helix 9 dimerization expands Bax pore assembly in membranes
Publication Title: The EMBO Journal -
Description Structural basis of nucleosome dynamics modulation by histone variants H2A.B and H2A.Z.2.2Min Zhou et al - The EMBO JournalEMBO J vol. 40, 04 January 2021
Article Title: Structural basis of nucleosome dynamics modulation by histone variants H2A.B and H2A.Z.2.2
Publication Title: The EMBO Journal -
Description A mixture of NAADP and IP3 evokes locally amplified Ca2+ release. (A) IP3 (15 μM in the intracellular pipette solution) evokes repetitive spikes of Ca2+‐sensitive current. The underlying Ca2+...
Article Title: Transformation of local Ca2+ spikes to global Ca2+ transients: the combinatorial roles of multiple Ca2+ releasing messengers
Publication Title: The EMBO Journal -
- Identifier
- embj2018101452-fig-0001ev
- License Type
- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description HEK cells transfected with STIM1‐CFP, Orai1‐mCherry, and ANO8 and treated with scrambled (black) or SARAF siRNA (red) were used to measure CRAC current with pipette solution containing 3 mM EGTA....
Article Title: Anoctamin 8 tethers endoplasmic reticulum and plasma membrane for assembly of Ca2+ signaling complexes at the ER/PM compartment
Publication Title: The EMBO Journal -
Description RFLP analyses to test for homoplasmy of Δycf6 chloroplast transformants. (A) Restriction analysis of total cellular DNA with EcoRI. Seven independently generated transplastomic lines, a wild‐type...
Article Title: Targeted inactivation of the smallest plastid genome‐encoded open reading frame reveals a novel and essential subunit of the cytochrome b6f complex
Publication Title: The EMBO Journal -
Description SIRT1 interacts with the RelA/p65 subunit of NF‐κB. (A) IP Westerns: H460 cells were transfected with either V5‐tagged SIRT1 or Flag‐tagged RelA/p65. Cell lysates were immunoprecipitated with the...
Article Title: Modulation of NF‐κB‐dependent transcription and cell survival by the SIRT1 deacetylase
Publication Title: The EMBO Journal -
- Identifier
- embj201797840-fig-0005ev
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Wide‐field time‐lapse images showing the recruitment of GFP‐LC3 to entotic vacuoles in wild‐type HCT116 cell‐in‐cell structures. Arrow indicates GFP‐LC3 recruitment to entotic vacuole. Asterisks...
Article Title: The WD40 domain of ATG16L1 is required for its non‐canonical role in lipidation of LC3 at single membranes
Publication Title: The EMBO Journal -
Description Crosslinking scheme uses introduced cysteines that are brought close enough to form disulfide bonds when Rad50 dimerizes with ATP. Crosslinking of Rad50cd‐2C variants (3.5 μM) with indicated...
Article Title: ATP‐driven Rad50 conformations regulate DNA tethering, end resection, and ATM checkpoint signaling
Publication Title: The EMBO Journal -
- Identifier
- embj2019102190-fig-0002
- License Type
- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description AMicropeptide CIP2A‐BP was immunostained with anti‐CIP2A‐BP antibody in MCF‐10A and MDA‐MB‐231 cells either mock‐treated or treated with TGF‐β. BMicropeptide CIP2A‐BP levels were determined by...
Article Title: Micropeptide CIP2A‐BP encoded by LINC00665 inhibits triple‐negative breast cancer progression
Publication Title: The EMBO Journal -
Description U2OS Flp‐In T‐REx cells were induced to express GFP‐CtIP‐wt or GFP‐CtIP‐K467A and transfected with CtIP siRNA. Cells were subsequently synchronized by thymidine incubation for 24 h and released for...
Article Title: APC/CCdh1 controls CtIP stability during the cell cycle and in response to DNA damage
Publication Title: The EMBO Journal -
Description Expression of chimeric proteins containing the cytoplasmic regions of Nav1.2 in hippocampal neurons. (A) Schematic representation of the neuronal sodium channel Nav1.2. The N‐ and C‐termini are...
Article Title: Identification of an axonal determinant in the C‐terminus of the sodium channel Nav1.2
Publication Title: The EMBO Journal -
Description Immunoblot and immunofluorescence analyses of Schwann cell cultures with antibodies to Kv‐channel α subunits. (A) Membrane fractions of mouse primary cultured SCs were subjected to immunoblot...
Article Title: Constitutive activation of delayed‐rectifier potassium channels by a Src family tyrosine kinase in Schwann cells
Publication Title: The EMBO Journal -
Description Detection of Ruk proteins in total protein extracted from adult rat tissues (A and B) and cell lines (C) by western blotting. (A) Shows elimination of specific bands after pre‐incubation of the...
Article Title: Negative regulation of PI 3‐kinase by Ruk, a novel adaptor protein
Publication Title: The EMBO Journal -
Description Protein–protein interactions of Sm15K and Sm16.5K proteins. (A) Immobilized proteins GST‐Sm15K (lanes 3, 4), GST‐Sm16.5K (lanes 5, 6), and as a control, GST (lanes 7, 8) were incubated with...
Article Title: Sm core variation in spliceosomal small nuclear ribonucleoproteins from Trypanosoma brucei
Publication Title: The EMBO Journal -
Description Chromatin structure of the niiA–niaD intergenic region in a wild‐type (areA+ nirA+) strain. (A) MNase digestion. To the right of the panel, the digestion pattern of the naked DNA is shown....
Article Title: The GATA factor AreA is essential for chromatin remodelling in a eukaryotic bidirectional promoter
Publication Title: The EMBO Journal -
Description TIM induction rescues features of the Drosophila activity cycle. (A) Activity profiles for heat‐shocked yw;tim01;hs‐tim flies. The animals were entrained for two LD cycles and then transferred to...
Article Title: TIMELESS‐dependent positive and negative autoregulation in the Drosophila circadian clock
Publication Title: The EMBO Journal -
Description Tapasin requirement for TAP‐dependent peptide loading in D.melanogaster cells. (A) Immunofluorescence and flow cytometry of SC2 cells transfected with Kb, β2m without tapasin (light line) or with...
Article Title: Retention of empty MHC class I molecules by tapasin is essential to reconstitute antigen presentation in invertebrate cells
Publication Title: The EMBO Journal -
Description H5 avian and H9 swine HA structures compared with H3 HA and HEF. (A) Ribbon diagram of the trimer of H5 (A/Dk/Sing/97) avian HA coloured by subdomains: receptor subdomain R (blue), vestigial enzyme...
Article Title: H5 avian and H9 swine influenza virus haemagglutinin structures: possible origin of influenza subtypes
Publication Title: The EMBO Journal -
- Identifier
- embj201796700-fig-0002ev
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Tsc1 was immunoprecipitated from HEK293 cell lysates using anti‐Tsc1 antibody and immunoblotted with indicated antibodies. IgG was used as control. These samples are the same as those presented in...
Article Title: Tumor suppressor Tsc1 is a new Hsp90 co‐chaperone that facilitates folding of kinase and non‐kinase clients
Publication Title: The EMBO Journal -
Description The UVC‐induced CREB and ATF‐1 phosphorylations are suramin resistant. (A) HeLa cells were pre‐incubated with suramin (0.3 mM) for 45 min where indicated (S) and then treated, with either UVC (30...
Article Title: CREB is activated by UVC through a p38/HOG‐1‐dependent protein kinase
Publication Title: The EMBO Journal -
- Identifier
- embj201695323-fig-0005
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Schematic display of a PTU. Nucleosome occupancy is plotted relative to the start codon (ATG) of the first gene of a PTU and averaged across all PTUs (n = 184). The definition of the first gene of...
Article Title: GT‐rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes
Publication Title: The EMBO Journal -
Description The (2Fo−Fc) electron density map contoured at the 1 σ level, following the inhibitor molecules Inh1 (−6 to −4) and Inh2 (+1 to +4) from the tunnel to the end of the open cleft. It shows as well...
Article Title: The crystal structure of the processive endocellulase CelF of Clostridium cellulolyticum in complex with a thiooligosaccharide inhibitor at 2.0 Å resolution
Publication Title: The EMBO Journal -
- Identifier
- embj201695223-fig-0004
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description ASchematic representation of the assay used to directly measure unhooking incisions. 32P‐labeled parental stands are indicated in red. Products before and after ICL unhooking during repair are...
Article Title: Recruitment and positioning determine the specific role of the XPF‐ERCC1 endonuclease in interstrand crosslink repair
Publication Title: The EMBO Journal -
Description Chromatin immunoprecipitation of ETO in Kasumi‐1, HL60, HL60‐PMA and HeLa cells with quantification of recovered DNA at eight points along the c‐FMS locus. The locations of the promoter, FIRE −1 kb...
Article Title: Epigenetic consequences of AML1–ETO action at the human c‐FMS locus
Publication Title: The EMBO Journal -
- Identifier
- embj201798083-fig-0002ev
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description An example region on chromosome 2 is shown for zygotic, two‐cell, and eight‐cell embryos, illustrating that in bulk Hi‐C data (Du et al, ; Ke et al, ), it is possible to identify enrichments of...
Article Title: A mechanism of cohesin‐dependent loop extrusion organizes zygotic genome architecture
Publication Title: The EMBO Journal -
Description KLF5 plays a key role in the activation of low‐grade PDAC enhancers, which activates epithelial transcriptional programmes, and suppresses ZEB1, a regulator of the high‐grade mesenchymal PDAC...
Article Title: The cis‐regulatory switchboard of pancreatic ductal cancer
Publication Title: The EMBO Journal -
- Identifier
- embj7601979-fig-0002
- License Type
- CC-BY-NC-ND
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Cystatin F is associated with cathepsin C in U937 and YT cells and inhibits its activity in vivo but not in vitro. (A) Proteins associated with anti‐cystatin F or control antibodies were isolated...
Article Title: Cystatin F is a cathepsin C‐directed protease inhibitor regulated by proteolysis
Publication Title: The EMBO Journal