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Displaying assets 1 - 30 of 60914 in total. Search results limited to 100 out of 2031 pages. Please refine your search query.
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Description Diagrams summarizing established binding sites in the myomesin molecule and arrangement of titin and myomesin in the M band. In (A), myosin and titin binding sites and phosphorylation sites in the...
Article Title: Molecular structure of the sarcomeric M band: mapping of titin and myosin binding domains in myomesin and the identification of a potential regulatory phosphorylation site in myomesin
Publication Title: The EMBO Journal -
Description phd2 is a hypoxia‐inducible gene. (A) Specificity of the anti‐PHD2 antibody. Western blot of total cell extracts of HeLa cells after 48 h of transfection with the indicated siRNAs (20 nM) using an...
Article Title: HIF prolyl‐hydroxylase 2 is the key oxygen sensor setting low steady‐state levels of HIF‐1α in normoxia
Publication Title: The EMBO Journal -
Description Cre‐mediated inducible expression of the μm heavy chain. (A) The B cell line J558Lmb‐1 was co‐transfected with the vectors pANMerCreMer and pSV2loxμm. In the latter vector, the reading frame of the...
Article Title: Induction of the antigen receptor expression on B lymphocytes results in rapid competence for signaling of SLP‐65 and Syk
Publication Title: The EMBO Journal -
Description A possible mechanism for regulation of competition between the RF2 and Sec‐tRNASec decoding molecules at the fdhF selenocysteine insertion site. (A) A kinetic scheme of the competition between the...
Article Title: A dynamic competition between release factor 2 and the tRNASec decoding UGA at the recoding site of Escherichia coli formate dehydrogenase H
Publication Title: The EMBO Journal -
Description Sensitivity of furanone C‐30‐treated P.aeruginosa biofilms to tobramycin. Scanning confocal laser microscopy (SCLM) photomicrographs of P.aeruginosa PAO1 biofilms grown in the absence (left panel)...
Article Title: Attenuation of Pseudomonas aeruginosa virulence by quorum sensing inhibitors
Publication Title: The EMBO Journal -
Description Gln63 as the main catalytic residue for Rap catalysis. (A) A bar diagram of the GAP activity of GAP1IP4BP and RASAL with Rap mutants, measured by reverse‐phase HPLC, except the Rap(T61Q) mutant...
Article Title: Unravelling the mechanism of dual‐specificity GAPs
Publication Title: The EMBO Journal -
Description Peroxisome retention and inheritance (A) free peroxisomes in the mother cell (stage I) are anchored to cortical ER by a tethering complex consisting of two molecules Pex3p, one located at the ER...
Article Title: Give what you can and keep what you need!
Publication Title: The EMBO Journal -
Description A–CFluorescence images (upper) and quantifications (lower) of GFP‐tagged CST‐1 (A, B) or GFP‐labeled F‐actin (C) and mCherry‐labeled plasma membrane and histones in WT and mig‐2 null‐mutant...
Article Title: Hippo kinases maintain polarity during directional cell migration in Caenorhabditis elegans
Publication Title: The EMBO Journal -
Description Expression pattern of ko in the muscle precursors of wild‐type (A, C and E) and KrCD+Kr1 (B and D) embryos and its alteration in response to ectopic Kr expression (F) shown by in situ...
Article Title: Krüppel target gene knockout participates in the proper innervation of a specific set of Drosophila larval muscles
Publication Title: The EMBO Journal -
Description Rb−/− cells contribute to chimeric embryo and adult brain. (A) XIST and Y‐chromosome paint FISH analysis on E13.5 and E15.5 embryo sections. In chimeras, male Rb−/− cells are marked with green...
Article Title: Cell‐autonomous and non‐cell‐autonomous functions of the Rb tumor suppressor in developing central nervous system
Publication Title: The EMBO Journal -
Description Multiple sequence alignment of some BRCT family members as obtained from Pfam v3.0 (Sonnhammer et al., 1997). The secondary structure of the XRCC1 BRCT domain is shown on top, labelled and coloured...
Article Title: Structure of an XRCC1 BRCT domain: a new protein–protein interaction module
Publication Title: The EMBO Journal -
Description hus1Δ yeast are supersensitive to bleomycin. Sensitivity of wild‐type (TE366) and hus1::LEU2 (TE484) yeast to bleomycin. The graph (A) shows viability (solid lines) and relative optical density...
Article Title: Hus1p, a conserved fission yeast checkpoint protein, interacts with Rad1p and is phosphorylated in response to DNA damage
Publication Title: The EMBO Journal -
Description Assembly of PulG pili in mutants lacking single minor pseudopilins. (A) PulG immunodetection in 0.005 OD600 nm units of cell and sheared fractions (C, SF) of E. coli carrying the pulG gene in a...
Article Title: Minor pseudopilin self‐assembly primes type II secretion pseudopilus elongation
Publication Title: The EMBO Journal -
Description ES‐specific lncRNAs are low abundance transcripts, and are probably regulated by pluripotent factors OCT4 and NANOG. (A) The expression levels of pluripotency lncRNAs were determined by qPCR in a...
Article Title: Human long non‐coding RNAs promote pluripotency and neuronal differentiation by association with chromatin modifiers and transcription factors
Publication Title: The EMBO Journal -
Description MuSK, α‐DB‐1, utrophin and VVAB4‐binding proteins are clustered by agrin in 1R– and 2R– myotubes. (A) Agrin‐treated myotubes were double‐labeled with rhodamine‐btx (a, e, i, m and q) and antibodies...
Article Title: Acetylcholine receptors are required for agrin‐induced clustering of postsynaptic proteins
Publication Title: The EMBO Journal -
Description Caspase‐3 is activated in Neuro‐2a cells after staurosporine treatment but not after thapsigargin treatment. (A) Staurosporine (10 nM) or thapsigargin (0.75 μM) was added to Neuro‐2a and, at the...
Article Title: NAIP interacts with hippocalcin and protects neurons against calcium‐induced cell death through caspase‐3‐dependent and ‐independent pathways
Publication Title: The EMBO Journal -
Description Sera from patients recovering from DENV‐4 infection contain antibodies that bind to the 5H2 epitope region on DI. (A, B) Indirect ELISAs using immobilized DENV‐4 sE (A) or rDI (B) and human serum...
Article Title: Structural insights into the neutralization mechanism of a higher primate antibody against dengue virus
Publication Title: The EMBO Journal -
Description 14‐3‐3γ stimulates p53 activity and G1 arrest. (A, B) Ecotopic expression of 14‐3‐3γ induces p21 dependently on p53. U2OS (panel A) or Saos 2 (panel B) cells were transfected with a combination of...
Article Title: 14‐3‐3γ binds to MDMX that is phosphorylated by UV‐activated Chk1, resulting in p53 activation
Publication Title: The EMBO Journal -
Description Comparison of chloroplast development in transgenic and wild‐type plants. Leaf sections of transgenic and wild‐type plants were examined by transmission electron microscopy. A transgenic plant with...
Article Title: A dynamin‐like protein in Arabidopsis thaliana is involved in biogenesis of thylakoid membranes
Publication Title: The EMBO Journal -
- Identifier
- embj201352-fig-0005
- License Type
- CC-BY-NC-ND
- Rights Holder
- Rights Managed by John Wiley & Sons
Description siRISC loading and siRNA‐mediated mRNA cleavage occur on the membrane of the rough endoplasmatic reticulum. (A) Membrane IPs were performed with HeLa lysates (as described in Materials and methods)...
Article Title: The rough endoplasmatic reticulum is a central nucleation site of siRNA‐mediated RNA silencing
Publication Title: The EMBO Journal -
- Identifier
- embj201695170-fig-0003
- License Type
- CC-BY-NC-ND
- Rights Holder
- Rights Managed by John Wiley & Sons
Description WT and SKO cells were treated with OA for 1 h and processed for ET. Representative single tomographic slices of ER–LD contacts. Orange arrowheads indicate narrow ER–LD contacts, and blue arrowheads...
Article Title: Seipin regulates ER–lipid droplet contacts and cargo delivery
Publication Title: The EMBO Journal -
Description Mg2+ dependence of the MgtE channel activity. (A–D) The MgCl2 concentration in the bath was increased from 0.2 to 1 mM and 5 to 10 mM, and single currents were measured at each MgCl2 concentration...
Article Title: Mg2+‐dependent gating of bacterial MgtE channel underlies Mg2+ homeostasis
Publication Title: The EMBO Journal -
Description Determination of GTPγS dissociation constants for 53K and the G domain of 50K. Filter binding assays were performed as described in Materials and methods. (A) and (C) represent the...
Article Title: The Escherichia coli trmE (mnmE) gene, involved in tRNA modification, codes for an evolutionarily conserved GTPase with unusual biochemical properties
Publication Title: The EMBO Journal -
Description Brg1 is required for the TGF‐β‐induced expression of lefty1 and nodal in vivo and binds to the lefty1 promoter. (A) Proteins from P19 cells transfected with individual siRNA duplexes against Brg1...
Article Title: Smads orchestrate specific histone modifications and chromatin remodeling to activate transcription
Publication Title: The EMBO Journal -
Description Operator binding of reduced and oxidized TetR variants analyzed by DNA retardation. DNA retardation analyses on 10% polyacrylamide gels are shown for wild‐type and each TetR variant as indicated on...
Article Title: Conformational changes necessary for gene regulation by Tet repressor assayed by reversible disulfide bond formation
Publication Title: The EMBO Journal -
Description (A) Other heterochromatin barrier sequences in S.cerevisiae. Plasmids containing the following S.cerevisiae sequences as barriers were tested as in Figure 1: STE6‐3′ (pRO506); SMK1‐3′ (pRO508);...
Article Title: RNA polymerase III and RNA polymerase II promoter complexes are heterochromatin barriers in Saccharomyces cerevisiae
Publication Title: The EMBO Journal -
- Identifier
- embj201696315-fig-0001ev
- License Type
- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description Confocal images showing cellular localization of various HyD‐LIR(X)‐GFP incorporating LIR motifs of different LC3‐binding proteins together with mRFP‐LC3B in MEFs upon autophagy induction (100 nM...
Article Title: Development of LC3/GABARAP sensors containing a LIR and a hydrophobic domain to monitor autophagy
Publication Title: The EMBO Journal -
Description The effect of DRB on transcriptional elongation in vivo.(A) In situ hybridization with intron‐1‐specific probes on homozygous transgenic day 11.5 fetal liver cells treated for 15 min with 100 μM...
Article Title: Chromatin interaction mechanism of transcriptional control in vivo
Publication Title: The EMBO Journal -
Description Assembly of the full‐length Sup35p into protein fibrils. (A) Dependence of the lag time preceding assembly on the concentration of Sup35p. Soluble Sup35p, 4 μM (•) and 6 μM (▪) was incubated...
Article Title: Molecular chaperones and the assembly of the prion Sup35p, an in vitro study
Publication Title: The EMBO Journal -
Description Schematic diagram that visualizes the interactions between RodZ and the MreBCD–MrdAB (PBP2 RodA) complexes of E. coli. RodZ is shown as a vertical bar with four domains. The HTH domain (magenta)...
Article Title: RodZ, a new player in bacterial cell morphogenesis
Publication Title: The EMBO Journal
Displaying assets 1 - 30 of 60914 in total. Search results limited to 100 out of 2031 pages. Please refine your search query.