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Displaying assets from 1 to 30 out of 9386
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- Identifier
- embj201797038-fig-0001
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Similarities in domain composition and amino acid sequence between murine DNMT3A and DNMT3B. Conserved amino acids are indicated as red bars. The longer and shorter DNMT3A isoforms DNMT3A1 and...
Article Title: Isoform‐specific localization of DNMT3A regulates DNA methylation fidelity at bivalent CpG islands
Publication Title: The EMBO Journal -
- Identifier
- embj201694914-fig-0004ev
- License Type
- CC-BY-NC-ND
- Rights Holder
- Creative Commons by John Wiley & Sons
Description MEF cells were transfected with PLIN1, wild‐type Mfn2, or Mfn2K109A as indicated. Then, 36 h later, cells were loaded with oleic acid (0.2 mM). After 12 h, protein extracts were obtained to test...
Article Title: Mfn2 is critical for brown adipose tissue thermogenic function
Publication Title: The EMBO Journal -
Description Complementation of dim‐2 mutation. (A) Physical map of cosmid X25:A8 and related DNA segments. The positions of the dim‐2 ORF (open box) and of restriction sites for NotI (N), EagI (E), Bsp120 I...
Article Title: dim‐2 encodes a DNA methyltransferase responsible for all known cytosine methylation in Neurospora
Publication Title: The EMBO Journal -
Description Complementation of a temperature‐sensitive CAK1 allele by either Csk1 or Mcs6–Mcs2 in budding yeast. The CAK1 ts strain (civ1‐4) was transformed by plasmids overexpressing csk1 (csk1‐p426), mcs6...
Article Title: Specificity of Cdk activation in vivo by the two Caks Mcs6 and Csk1 in fission yeast
Publication Title: The EMBO Journal -
- Identifier
- embj2019101955-fig-0001
- License Type
- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description A–CSnapshots of ChIP‐seq signals of Sen1 (this study), RNAP3 (this study) and RNAP2 (data from Larochelle et al ) across (A) a 25‐kb region of chromosome 3 containing several tRNA and 5S rRNA...
Article Title: Senataxin homologue Sen1 is required for efficient termination of RNA polymerase III transcription
Publication Title: The EMBO Journal -
- Identifier
- embj201797876-fig-0005ev
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Weighted chemical shift perturbation analysis for 15N‐labelled wt Ub, Ub mutants and Parkin Ubl in the presence of PhPINK1. Isothermal calorimetry titration analysis of wt or TVLN Ub into PhPINK1....
Article Title: An invisible ubiquitin conformation is required for efficient phosphorylation by PINK1
Publication Title: The EMBO Journal -
Description (A and D) The agravitropic root growth phenotype of the auxin response mutants axr2 and axr3 cannot be rescued by 1‐NAA. The axr2‐1 and axr3‐1 mutants were germinated on either hormone‐free medium...
Article Title: AUX1 regulates root gravitropism in Arabidopsis by facilitating auxin uptake within root apical tissues
Publication Title: The EMBO Journal -
- Identifier
- embj2012227-fig-0003
- License Type
- CC-BY-NC-ND
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Bone lesions in Sca1‐MafB mice. (A) Detection of osteolytic lesions and pathological fractures (indicated by orange arrows) by X‐ray analysis of Sca1‐MafB mice. A total number of nine mice (between...
Article Title: A novel molecular mechanism involved in multiple myeloma development revealed by targeting MafB to haematopoietic progenitors
Publication Title: The EMBO Journal -
Description Bar charts showing the relative surface of clones attached and not attached between 3 and 24 weeks after induction at P21 or P42. Data are represented as mean ± s.e.m. Computer simulation on 2D...
Article Title: Bimodal behaviour of interfollicular epidermal progenitors regulated by hair follicle position and cycling
Publication Title: The EMBO Journal -
- Identifier
- embj201696025-fig-0003ev
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Ingenuity pathway interaction analysis of nuclear proteins that show significant transcriptional upregulation in stimulated MDM compared to unstimulated MDM. The figure demonstrates a single...
Article Title: A G1‐like state allows HIV‐1 to bypass SAMHD1 restriction in macrophages
Publication Title: The EMBO Journal -
- Identifier
- embj201696127-fig-0001ev
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Estimation of Salmonella RaiZ copy number throughout growth. Total RNA from ~7 × 108 cells was loaded in each lane along with defined amounts of in vitro‐synthesized MS2‐RaiZ (Smirnov et al, ) and...
Article Title: Molecular mechanism of mRNA repression in trans by a ProQ‐dependent small RNA
Publication Title: The EMBO Journal -
Description A, BSingle‐molecule analysis of DNA replication by DNA combing of siRNA‐transfected cells pulse‐labelled with IdU (10 min) and CIdU (20 min). (A) Distribution of inter‐track distances and (B) size...
Article Title: BRPF3‐HBO1 regulates replication origin activation and histone H3K14 acetylation
Publication Title: The EMBO Journal -
- Identifier
- embj2009271-fig-0004
- License Type
- CC-BY-NC-SA
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Analysis of histone methylation and differentiation in Jmjd3−/− macrophages. Macrophages derived from the foetal livers of Jmjd3−/− mice were analysed for Jmjd3 protein and mRNA expression (A) and...
Article Title: Jmjd3 contributes to the control of gene expression in LPS‐activated macrophages
Publication Title: The EMBO Journal -
- Identifier
- embj2018100928-fig-0001ev
- License Type
- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description Lentiviral construct to drive co‐expression of luciferase 2 and EGFP (separated by a P2A peptide). Stable expression is selectable by the puromycin resistance marker. Additional features are...
Article Title: 3D model for CAR‐mediated cytotoxicity using patient‐derived colorectal cancer organoids
Publication Title: The EMBO Journal -
- Identifier
- embj201796797-fig-0001
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Oxygen consumption rate in AD fibroblasts (FAD). Oxygen consumption rate in PS‐DKO MEFs. Oxygen consumption rate in SH‐SY5Y cells treated with DAPT, an inhibitor of γ‐secretase activity. Oxygen...
Article Title: Increased localization of APP‐C99 in mitochondria‐associated ER membranes causes mitochondrial dysfunction in Alzheimer disease
Publication Title: The EMBO Journal -
- Identifier
- embj201797876-fig-0004ev
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Differential scanning calorimetry endotherms of indicated Ub variants in unphosphorylated and phosphorylated forms. A table summarising fitted melting parameters of each species is shown. Similar...
Article Title: An invisible ubiquitin conformation is required for efficient phosphorylation by PINK1
Publication Title: The EMBO Journal -
- Identifier
- embj201695621-fig-0002ev
- License Type
- CC-BY-NC-ND
- Rights Holder
- Creative Commons by John Wiley & Sons
Description MNase‐seq nucleosomal DNA midpoint mapping with and without Hsf1 for all 11 SAGA‐dominated targets (orange) and all 10 TFIID‐dominated targets (blue). The arrow denotes the transcriptional start...
Article Title: Molecular mechanisms that distinguish TFIID housekeeping from regulatable SAGA promoters
Publication Title: The EMBO Journal -
- Identifier
- embj201695751-fig-0001ev
- License Type
- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description Venn diagrams of the number of promoters targeted by AbdA (left) and Ubx (right) in S2 cells (blue), Kc167 cells (pink) and embryos (green) show that AbdA targets 6,011 identical promoters in S2...
Article Title: The Hox proteins Ubx and AbdA collaborate with the transcription pausing factor M1BP to regulate gene transcription
Publication Title: The EMBO Journal -
Description Docking into a motor–microtubule cryoEM map. The new atomic structure of Ncd is shown docked into a cryoEM density map of nucleotide‐free Ncd bound to microtubules (Hirose et al., 1998, 1999). (A)...
Article Title: Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd
Publication Title: The EMBO Journal -
- Identifier
- embj201694248-fig-0006
- License Type
- CC-BY-NC-ND
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Chitin‐induced MAPK activation in a mapkkk5 mutant (Line 1) expressing MAPKKK56xA. MAPK activity was detected using immunoblots with α‐pMAPK. Chitin‐induced callose deposition in the MAPKKK56xA...
Article Title: The Arabidopsis CERK1‐associated kinase PBL27 connects chitin perception to MAPK activation
Publication Title: The EMBO Journal -
- Identifier
- embj201592116-fig-0002ev
- License Type
- CC-BY-NC-ND
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Genomic sequence proximal to FoxA2. Tcf7l1 binding peak as in Fig A with regions conserved within eutherian mammals shown in pink boxes below (Flicek et al, ). 250‐bp sequence of the Tcf7l1‐binding...
Article Title: Convergence of cMyc and β‐catenin on Tcf7l1 enables endoderm specification
Publication Title: The EMBO Journal -
Description View onto the presumable membrane‐interacting (inner) surface involving the wing and the β‐roll domains of each protomer. The β‐roll domains are pink (protomer A) and red (protomer B), the wing...
Article Title: Zika virus NS1, a pathogenicity factor with many faces
Publication Title: The EMBO Journal -
- Identifier
- embj2018101051-fig-0003
- License Type
- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description VGLL4 mRNA level was measured by RT–qPCR in C57BL/6J mice TA muscles. Samples were collected from cardiotoxin (CTX)‐injected TA muscles at 0, 1, 2, 3, 4, 5, 6, 7, 14 days post‐injury (n = 6 mice)....
Article Title: Dual function of VGLL4 in muscle regeneration
Publication Title: The EMBO Journal -
- Identifier
- embj2008169-fig-0001
- License Type
- CC-BY-NC-ND
- Rights Holder
- Creative Commons by John Wiley & Sons
Description The PI kinases and phosphatases and their genetic defects. (A) Schematic representation of the PI metabolic cycle with the PIKs indicated in blue, and the PI phosphatases in green. (B) Listing of...
Article Title: Function and dysfunction of the PI system in membrane trafficking
Publication Title: The EMBO Journal -
- Identifier
- embj201352-fig-0002
- License Type
- CC-BY-NC-ND
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Excess of inactive siRNA masks active population. (A, B) Ago2 IPs were performed in a quantitative manner 24 h after the HeLa cells were transfected with SSB siRNA. mRNA levels were determined by...
Article Title: The rough endoplasmatic reticulum is a central nucleation site of siRNA‐mediated RNA silencing
Publication Title: The EMBO Journal -
Description Tyrosine phosphorylation of p34cdc2 is required for correct conidiophore morphogenesis. A wild‐type strain (A and D) and a nimXcdc2AF strain (FRY‐20‐1) (B, C and E) were induced to undergo...
Article Title: Interaction between developmental and cell cycle regulators is required for morphogenesis in Aspergillus nidulans
Publication Title: The EMBO Journal -
Description Mycobacterium tuberculosis infection of macrophages induces the expression of nuclear SUV39H1 and relocalization of the protein to the host cell surface. At the plasma membrane, SUV39H1...
Article Title: Moonlighting activity of the epigenetic machinery restrains infection
Publication Title: The EMBO Journal -
- Identifier
- embj2011346-fig-0004
- License Type
- CC-BY-NC-ND
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Manipulation of NetrinG–NGL recognition by structure‐guided grafting of specificity. (A) SPR experiments for the interaction of WT NetrinG1Lam–EGF1/4 and NetrinG2Lam–EGF1 with WT NGL1/2LRR–Ig...
Article Title: Structural basis for cell surface patterning through NetrinG–NGL interactions
Publication Title: The EMBO Journal -
Description A–DGastric stem cell proliferation was measured by morphometric analysis of GFP/Ki67 cell numbers in Notch‐inhibited or Notch‐activated antral tissue, showing decreased LGR5‐GFP stem cell...
Article Title: Notch signaling regulates gastric antral LGR5 stem cell function
Publication Title: The EMBO Journal -
- Identifier
- embj201593260-fig-0002ev
- License Type
- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description Expression levels as well as changes detected in PR, ERK2, HP1γ, LSD1, and H3K9me3 after hormone were calculated for the control genes TFAP2B, BAZ2B, and the non‐regulated genes RABEPK and PELO....
Article Title: Hormone‐induced repression of genes requires BRG1‐mediated H1.2 deposition at target promoters
Publication Title: The EMBO Journal