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Displaying assets from 1 to 30 out of 18405
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- Identifier
- embj201284188-fig-0005
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description A–DWestern blot analysis of complex glycosylated TRPV5 (A) protein (92 kDa) expression in renal cortical total membrane fractions, as well as phosphorylated ERK1/2 (B), phosphorylated SGK1 (C), and...
Article Title: FGF23 promotes renal calcium reabsorption through the TRPV5 channel
Publication Title: The EMBO Journal -
- Identifier
- embj201164-fig-0005
- License Type
- CC-BY-NC-SA
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Model of the complete yeast RNAP II–Spt4/5 elongation complex. (A) Model of the complete yeast RNAP II elongation complex with bound Spt4/5. Proteins are shown as molecular surfaces with key...
Article Title: Architecture of the RNA polymerase–Spt4/5 complex and basis of universal transcription processivity
Publication Title: The EMBO Journal -
- Identifier
- embj2008175-fig-0007
- License Type
- CC-BY-NC-SA
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Depletion of CENP‐V by RNA interference affects the distribution of pericentromeric heterochromatin. (A, A′) Distribution of heterochromatin stained with anti‐H3K9me3 in nucleoplasmic foci in HeLa...
Article Title: CENP‐V is required for centromere organization, chromosome alignment and cytokinesis
Publication Title: The EMBO Journal -
Description Comparison of the localisation of peaks called with unique genomic regions of AbdA (S2 and Kc167 cells) and Ubx (S2 cells, Kc167 cells and embryos). Heatmap representation of the enrichment of AbdA...
Article Title: The Hox proteins Ubx and AbdA collaborate with the transcription pausing factor M1BP to regulate gene transcription
Publication Title: The EMBO Journal -
Description Tap‐p15, Thoc5 and Aly constitute a hetero‐tetramer. (A) Purified Thoc5–FLAG (25 μg) along with increasing amounts of full‐length Tap‐p15 (lanes 2–4; 0, 8, 16 μg) was incubated with GSH‐beads...
Article Title: Adaptor Aly and co‐adaptor Thoc5 function in the Tap‐p15‐mediated nuclear export of HSP70 mRNA
Publication Title: The EMBO Journal -
Description Model of the transmembrane region of the proposed 2:2 complex of NpSRII with its transducer NpHtrII (view from cytoplasm). The structure of NpSRII (orange) is based on a BR‐crystal structure (Essen...
Article Title: Structural insights into the early steps of receptor—transducer signal transfer in archaeal phototaxis
Publication Title: The EMBO Journal -
- Identifier
- embj201164-fig-0001
- License Type
- CC-BY-NC-SA
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Spt4/5 binds endogenous RNAPs and a recombinant RNAP clamp. (A) Yeast (S. cerevisiae) complexes. SDS–PAGE analysis (Coomassie staining) of purified recombinant Spt4/5 (containing full‐length Spt4...
Article Title: Architecture of the RNA polymerase–Spt4/5 complex and basis of universal transcription processivity
Publication Title: The EMBO Journal -
Description Proliferation of lines A and B in liquid cultures supplemented with recombinant hematopoietic growth factors. Cells (104 per well) from each line were cultured with the indicated cytokines and cell...
Article Title: Immortalization and leukemic transformation of a myelomonocytic precursor by retrovirally transduced HRX–ENL
Publication Title: The EMBO Journal -
- Identifier
- embj2020105499-fig-0002ev
- License Type
- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description Super‐resolution images of primary cilia. NIH3T3 cells serum‐starved for 48 h to induce primary cilia were immunostained and imaged through Three Dimensional Structured Illumination Microscopy...
Article Title: Rabl2 GTP hydrolysis licenses BBSome‐mediated export to fine‐tune ciliary signaling
Publication Title: The EMBO Journal -
Description The D1 but not the D2 kinase domain of p90rsk is required for phosphorylation of Myt1. (A) Schematic representation of the lysine to arginine mutations introduced in p90rsk. (B) In vitro...
Article Title: A link between MAP kinase and p34cdc2/cyclin B during oocyte maturation: p90rsk phosphorylates and inactivates the p34cdc2 inhibitory kinase Myt1
Publication Title: The EMBO Journal -
Description Association of Fes with PlexA1 and its negative regulation by NP‐1 co‐expression in COS‐7 cells. (A) COS‐7 cells were transfected with the indicated expression vectors and total cell lysates were...
Article Title: Involvement of Fes/Fps tyrosine kinase in semaphorin3A signaling
Publication Title: The EMBO Journal -
Description Representative FACS plots of freshly isolated epidermal cells (Epi d0) and day 14 (d14) cultures of epidermal cells in different growth conditions and media. KGM 2D: 2D in basal KGM; 3C:...
Article Title: Hair follicle stem cell cultures reveal self‐organizing plasticity of stem cells and their progeny
Publication Title: The EMBO Journal -
- Identifier
- embj2019103365-fig-0004ev
- License Type
- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description A–CCryo‐EM density maps filtered according to local resolution used to build molecular models. (A) Cryo‐EM map of the pre‐state RNC with tRNA in the A site. (B) Cryo‐EM map of the CGA–CCG‐stalled...
Article Title: Molecular mechanism of translational stalling by inhibitory codon combinations and poly(A) tracts
Publication Title: The EMBO Journal -
Description Abnormal migration of mesodermal precursors and pole cells in mutant embryos. Wild‐type embryos (A, C) or embryos derived from Rap1/Rap1 germline clones (B, D) have been stained with anti‐Twist and...
Article Title: The Rap1 GTPase functions as a regulator of morphogenesis in vivo
Publication Title: The EMBO Journal -
- Identifier
- embj201592116-fig-0002
- License Type
- CC-BY-NC-ND
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Assay of Tcf7l1 mRNA by RT–PCR for the first 4 days of differentiation in the presence or absence of 3 μM CH. Average and SD of three independent experiments. Day 2: *P = 0.048, Day 3: *P = 0.036....
Article Title: Convergence of cMyc and β‐catenin on Tcf7l1 enables endoderm specification
Publication Title: The EMBO Journal -
Description Effects of SIKE on assembly of the TRIF‐IKKε‐IRF‐3, TRIF‐TBK1‐IRF3 and TRIF‐TRAF6‐RIP complexes. (A) SIKE disrupts the TRIF‐IKKε‐IRF‐3 complex. The 293 cells (1 × 106) were transfected with 5 μg of...
Article Title: SIKE is an IKKε/TBK1‐associated suppressor of TLR3‐ and virus‐triggered IRF‐3 activation pathways
Publication Title: The EMBO Journal -
- Identifier
- embj201592337-fig-0001
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Ubiquitination assays show wild‐type and UblR0RBR parkin are inhibited for chain formation (top) and Miro1 ubiquitination (bottom) in the absence of phosphoubiquitin at zero time point and in the...
Article Title: Disruption of the autoinhibited state primes the E3 ligase parkin for activation and catalysis
Publication Title: The EMBO Journal -
Description CpG more potently activates Smad3/4 than polyI:C through TGF‐β. (A) BMMs were stimulated with CpG (100 nM), polyI:C (1 μg/ml) or TGFβ1 (2.5 ng/ml). Nuclear extract was collected and analyzed by...
Article Title: TLR agonists regulate PDGF‐B production and cell proliferation through TGF‐β/type I IFN crosstalk
Publication Title: The EMBO Journal -
Description Loss of GnT‐V expression does not phenocopy SPPL3 overexpression. HEK293 cells transiently transfected with non‐targeting (ctrl), SPPL3‐specific or MGAT5‐specific siRNA pools (20 nM each), and...
Article Title: Shedding of glycan‐modifying enzymes by signal peptide peptidase‐like 3 (SPPL3) regulates cellular N‐glycosylation
Publication Title: The EMBO Journal -
Description DNA‐PK–p53 complex formation in apoptotic cells. (A) p53 co‐immunoprecipitates with DNA‐PK. MEFs or MEFs expressing E1A were either untreated (CTRL) or γ‐irradiated (IR). Cell extracts were then...
Article Title: DNA damage‐induced apoptosis requires the DNA‐dependent protein kinase, and is mediated by the latent population of p53
Publication Title: The EMBO Journal -
- Identifier
- embj2010315-fig-0007
- License Type
- CC-BY-NC-SA
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Charge neutralization in the hinge channel does not change cohesin's stable association with chromatin, but reduces acetylation of Smc3AAA protein. (A) Smc3AAA‐GFP/Smc1DD complex associates stably...
Article Title: A positively charged channel within the Smc1/Smc3 hinge required for sister chromatid cohesion
Publication Title: The EMBO Journal -
- Identifier
- embj2019101597-fig-0001ev
- License Type
- Rights Managed
- Rights Holder
- John Wiley & Sons, Inc.
Description Heatmap of RNA‐seq data for C. albicans homologs to “meiotic” genes in diploid and tetraploid cells on YPD and PRE‐SPO media at 24 h, including programmed formation of DNA double‐strand breaks...
Article Title: Metabolism‐induced oxidative stress and DNA damage selectively trigger genome instability in polyploid fungal cells
Publication Title: The EMBO Journal -
Description Differential surface distribution of CD4 chimeras containing the C‐terminal region of Nav1.1, Nav1.2 and Nav1.6
Article Title: Identification of an axonal determinant in the C‐terminus of the sodium channel Nav1.2
Publication Title: The EMBO Journal -
Description (A) Constitutive activation of the NF‐κB complex in Notch3 tg(+) thymocytes. EMSA of NF‐κB complexes in nuclear extracts from freshly isolated thymocytes of 3‐week‐old tg(+) (tg) or wild‐type (wt)...
Article Title: Constitutive activation of NF‐κB and T‐cell leukemia/lymphoma in Notch3 transgenic mice
Publication Title: The EMBO Journal -
Description In vitro transport experiments using purified native kinesin from porcine brains and recombinant mNUDC. (A) Immunoabsorption of endogenous mNUDC by an anti‐mNUDC antibody. We found a substantial...
Article Title: mNUDC is required for plus‐end‐directed transport of cytoplasmic dynein and dynactins by kinesin‐1
Publication Title: The EMBO Journal -
- Identifier
- embj201387369-fig-0006
- License Type
- CC-BY-NC-ND
- Rights Holder
- Creative Commons by John Wiley & Sons
Description A–CDDK‐dependent recruitment of firing factors, RPC components and other replication factors to reconstituted pre‐RCs. Note that the y‐axis is log10‐transformed. Thus, each unit represents a...
Article Title: Prereplicative complexes assembled in vitro support origin‐dependent and independent DNA replication
Publication Title: The EMBO Journal -
- Identifier
- embj201796821-fig-0002
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description Fmn2 expression was analyzed via qPCR in brain regions from 3‐month‐old mice (left panel, n = 5) and in mouse primary hippocampal neurons, astrocytes (n = 5) and human post‐mortem hippocampus...
Article Title: Formin 2 links neuropsychiatric phenotypes at young age to an increased risk for dementia
Publication Title: The EMBO Journal -
- Identifier
- embj201591973-fig-0004
- License Type
- CC-BY
- Rights Holder
- Creative Commons by John Wiley & Sons
Description A, BLM2 cells transfected with siRNA targeting PTPRN2 (A), PLCβ1 (B) or a control siRNA were transfected with Lyn11‐FRB and INPP5E‐FKBP, treated with either DMSO or 100 nM rapamycin and subjected...
Article Title: PTPRN2 and PLCβ1 promote metastatic breast cancer cell migration through PI(4,5)P2‐dependent actin remodeling
Publication Title: The EMBO Journal -
Description Three‐dimensional reconstruction of TPP II from D.melanogaster. (A) Surface representation of the complex. (1–3) Rotation of the TPP II reconstruction in tilted dumb‐bell orientation around its...
Article Title: A giant protease with a twist: the TPP II complex from Drosophila studied by electron microscopy
Publication Title: The EMBO Journal -
Description Mapping the DNA‐PK and ATM phosphorylation sites in Artemis. (A) A schematic of Artemis indicating the DNA‐PK phosphorylation sites (underlined sites were identified by MS). (B) Purified DNA‐PK or...
Article Title: DNA‐PK autophosphorylation facilitates Artemis endonuclease activity
Publication Title: The EMBO Journal