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SV40 expression of wild cADMSCs and immortalized cADMSCs. A, Quantitative real‐time PCR, (P, Plasmid pLOX‐Ttag‐ires‐TK, T, immortalized cADMSCs, W, wild cADMSCs); B, Western blot; and C, immunocytochemistry. bar = 200 µm. D, Morphology of wild cADMSCs and immortalized cADMSCs. Immortalized cADMSCs were maintained in a short spindle shape, similar to the third passage of wild cADMSCs

Credit:
Fang, J., Wei, Y., Teng, X., Zhao, S., Hua, J., Choi, J., Yong, K., Wan Safwani, W., Varghese, J., Griffin, M., Mosahebi, A., Butler, P., Cyranoski, D., Ozkul, Y., Galderisi, U., Wei, Y., Fang, J., Cai, S., Lv, C., Zhang, S., Hua, J., Li, N., Pan, S., Zhu, H., Mu, H., Liu, W., Hua, J., Wang, F., Liu, C., Zhang, S., Harasymiak‐Krzyżanowska, I., Niedojadło, A., Karwat, J., Tátrai, P., Szepesi, Á., Matula, Z., Wang, L., Song, K., Qu, X., Moon, H., Yoon, S., Hur, Y., Balducci, L., Blasi, A., Saldarelli, M., Alwin Prem Anand, A., Gowri Sankar, S., Kokila Vani, V., Land, H., Parada, L., Weinberg, R., Lu, S., Wang, J., & Ye, J. (2018). Immortalization of canine adipose‐derived mesenchymal stem cells and their seminiferous tubule transplantation. Journal of Cellular Biochemistry, 119(4), 3663-3670. https://doi.org/10.1002/jcb.26574.