Asset Details
- Identifier:
- pim12091-fig-0001
- Description:
- The role of anti‐Smteg antibodies in S. mansoni elimination. Newly transformed schistosomula were incubated with sera from mice inoculated with saline + CFA/IFA (a–b) or immunized with Schistosoma mansoni schistosomula tegument (Smteg) + CFA/IFA (c–d). Antibody reactivity to surface schistosomula proteins were detected by a secondary anti‐mouse IgG‐FITC‐conjugated antibody, the mean fluorescence intensity in the schistosomula tegument after incubation with sera from mice immunized with saline or with Smteg and anti‐IgG‐FITC‐conjugated antibody was determined using ImageJ in four schistosomula per treatment (e). Significant differences between treatments determined by Mann–Whitney test are pointed in the graphic (e). Complement activation and cytotoxicity in schistosomula were assessed in vitro (f). The viability of schistosomula was observed by fluorescence microscopy. Bars represent the percentage of dead schistosomula of two independent experiments + SD. Three wells per treatment were analysed in each experiment. Significant differences in the percentage of dead parasite in comparison with wells that contain schistosomula with medium are denoted by an asterisk (P < 0·05). Other statistically significant differences are pointed in the graphic. Post‐challenge infection determination of worm burden (g), eggs number in the intestine (h) or liver (i) from mice that received intravenous transference of sera from saline inoculated animals (white bars) and Smteg‐immunized animals (black bars). Grey bars represent the results from challenge infection control group. Bars represent the mean values ± SD of two independent assays with 15 animals for group in each assay. Statistically significant differences between groups are pointed in the graphic.
- License:
- Rights Managed
- Rights Holder:
- John Wiley & Sons, Inc.
- License Rights Holder:
- Copyright © 2014 John Wiley & Sons Ltd
- Asset Type:
- Image
- Asset Subtype:
- Chart/Graph
- Image Orientation:
- Landscape
- Image Dimensions:
- x
- Image File Size:
- 304 KB
- Creator:
- T. T. Melo, I. C. Sena, N. Araujo, C. T. Fonseca
- Credit:
- Melo, T. T., Sena, I. C., Araujo, N., & Fonseca, C. T. (2014). Antibodies are involved in the protective immunity induced in mice by Schistosoma mansoni schistosomula tegument (Smteg) immunization. Parasite Immunology, 36(2), 107-111..
- Collection:
- Keywords:
- Restrictions:
- Property Release:
- No
- Model Release:
- No
- Purchasable:
- Yes
- Sensitive Materials:
- No
- Article Title:
- Antibodies are involved in the protective immunity induced in mice by Schistosoma mansoni schistosomula tegument (Smteg) immunization
- Article Authors:
- T. T. Melo, I. C. Sena, N. Araujo, C. T. Fonseca
- Article Copyright Year:
- 2014
- Publication Title:
- Parasite Immunology
- Publication Volume:
- 36
- Publication Issue:
- 2
- Publication Date:
- 02/01/2014
- DOI:
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