Asset Details
- Identifier:
- pim12788-fig-0007
- Description:
- Comparative serum antibody responses to recombinant Pso‐EIP‐1 and rPso o 2 across a six‐week time course of infestation with Psoroptes ovis. (A) To compare serum IgG responses to bacterially expressed recombinant Pso‐EIP‐1 and Pso o 2 proteins, both antigens were used in ELISA with sera from individual sheep (n = 7) infested over a 6‐week time course of infestation with P. ovis, including pre‐infestation sera from the same animals taken 1 wk prior to infestation (−1 wpi). In both cases, the serum samples were diluted 1/400 in sample buffer (SB) prior to use in the ELISA. Based on the optimum OD450nm cut‐off values defined from the ROC analysis, we determined the number of animals classified as positive for sheep scab at each time point post‐infestation for each of the antigens; (B) Pso‐EIP‐1 and (C) Pso o 2. Horizontal red line indicates optimum OD450nm cut‐off for each assay based on maximum values for sensitivity and specificity (rPso‐EIP‐1 ≥0.9035; rPso o 2 ≥0.8002). NB: Not all serum samples were available for each of the seven animals at each timepoint; however, the following numbers were used in the ELISA (−1 wpi = 7/7; 1 wpi = 7/7; 2 wpi = 7/7; 3 wpi = 7/7; 4 wpi = 5/7; 5 wpi = 4/7; 6wpi = 7/7)
- License:
- Rights Managed
- Rights Holder:
- John Wiley & Sons, Inc.
- License Rights Holder:
- Copyright © 2020 John Wiley & Sons Ltd
- Asset Type:
- Image
- Asset Subtype:
- Chart/Graph
- Image Orientation:
- Portrait
- Image Dimensions:
- 1992 x 4008
- Image File Size:
- 196 KB
- Creator:
- Stewart T. G. Burgess, Francesca Nunn, Kath Bartley, David Frew, Kevin McLean, Neil F. Inglis, Kara McGeachy, Michael E. Taliansky, Andrew J. Love, Andrew J. Nisbet
- Credit:
- Burgess, S., Nunn, F., & Bartley, K. (2020). Psoroptes ovis‐Early Immunoreactive Protein (Pso‐EIP‐1) a novel diagnostic antigen for sheep scab. Parasite Immunology, 42(12), n/a. https://doi.org/10.1111/pim.12788.
- Collection:
- Keywords:
- Restrictions:
- Property Release:
- No
- Model Release:
- No
- Purchasable:
- Yes
- Sensitive Materials:
- No
- Article Title:
- Psoroptes ovis‐Early Immunoreactive Protein (Pso‐EIP‐1) a novel diagnostic antigen for sheep scab
- Article Authors:
- Stewart T. G. Burgess, Francesca Nunn, Kath Bartley, David Frew, Kevin McLean, Neil F. Inglis, Kara McGeachy, Michael E. Taliansky, Andrew J. Love, Andrew J. Nisbet
- Article Copyright Year:
- 2020
- Publication Title:
- Parasite Immunology
- Publication Volume:
- 42
- Publication Issue:
- 12
- Publication Date:
- 12/01/2020
- DOI:
- https://doi.org/10.1111/pim.12788
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