Asset Details

  • Description:
  • Composition and interchange of Hsp90‐based chaperone complexes. Schematic diagram showing the composition of, and transition between the 'intermediate' and 'mature' Hsp90 chaperone complexes, incorporating the current literature plus the new data presented here. The client protein is not shown. The 'intermediate' complex, which lasts around 2–3 min post‐translation of the client protein (Smith, 1993; Smith et al., 1995), contains an Hsp90 dimer–Sti1 dimer complex, with access to the ATP‐binding sites in the N‐terminal domains of the Hsp90, blocked by Sti1. The number of Hsp70 (plus associated Hsp40 and Hips) in this complex remains to be determined. Displacement of Sti1 and Hsp70 etc. unblocks the ATP‐binding site and allows binding of other TPR‐domain co‐chaperones and p23/Sba1, forming 'mature' complexes whose lifetime is ∼5 min. The number of p23/Sba1 molecules bound per 'mature' complex has not been determined. Geldanamycin blocks ATP binding and prevents the conversion into the 'mature' complex. Hydrolysis of bound ATP by Hsp90 releases p23/Sba1 and the client protein. Subsequent re‐binding of Sti1 would promote release of ADP and immunophilins, priming the Hsp90 complex for binding of a new client protein molecule delivered into the 'intermediate' complex by Hsp70 (plus Hsp40 and Hip).
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • Copyright © 2013 Wiley Periodicals, Inc
  • Asset Type:
  • Image
  • Asset Subtype:
  • Chart/Graph
  • Image Orientation:
  • Portrait
  • Image Dimensions:
  • 424 x 981
  • Image File Size:
  • 55.6 KB
  • Creator:
  • Chrisostomos Prodromou, Giuliano Siligardi, Ronan O'Brien, Derek N. Woolfson, Lynne Regan, Barry Panaretou, John E. Ladbury, Peter W. Piper, Laurence H. Pearl
  • Credit:
  • Prodromou, C., Siligardi, G., O'Brien, R., Woolfson, D. N., Regan, L., Panaretou, B., Ladbury, J. E., Piper, P. W., & Pearl, L. H. (1999). Regulation of Hsp90 ATPase activity by tetratricopeptide repeat (TPR)‐domain co‐chaperones. The EMBO Journal, 18(3), 754-762..
  • Collection:
  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Chrisostomos Prodromou, Giuliano Siligardi, Ronan O'Brien, Derek N. Woolfson, Lynne Regan, Barry Panaretou, John E. Ladbury, Peter W. Piper, Laurence H. Pearl
  • Article Copyright Year:
  • 1999
  • Publication Volume:
  • 18
  • Publication Issue:
  • 3
  • Publication Date:
  • 02/01/1999
  • DOI:

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