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MiR‐99a‐5p was downregulated in breast cancer tissues, whereas CDC25A was upregulated. (a) The miR‐99a‐5p levels in breast cancer cells and breast cells were determined by qRT‐PCR; miR‐99a‐5p was downregulated in breast cancer cells. (b) The miR‐99a‐5p levels in breast cancer tissues and adjacent tissues were determined by qRT‐PCR; miR‐99a‐5p was downregulated in breast cancer tissues. (c) We conducted prognostic analysis on miR‐99a‐5p; upregulated miR‐99a‐5p had a good prognosis. (d) The CDC25A levels in breast cancer cells and breast cells were determined by Western blot analysis; CDC25A was upregulated in breast cancer cells. (e) The CDC25A protein levels in breast cancer tissues and adjacent tissues were detected by Western blot analysis; the expression of CDC25A was upregulated in tumor tissues. (f) The expressions of miR‐99a‐5p and CDC25A were negative‐related. (g) According to the CDC25A prognostic analysis, patients with a low CDC25A expression held a higher survival rate. Student's t test and Pearson correlation coefficient were used to analyze the significant differences. *p < 0.05, **p < 0.01, compared with the NC group. qRT‐PCR: quantitative real‐time polymerase chain reaction; NC: negative control [Color figure can be viewed at wileyonlinelibrary.com]

Credit:
Qin, H., & Liu, W. (2019). MicroRNA‐99a‐5p suppresses breast cancer progression and cell‐cycle pathway through downregulating CDC25A. Journal of Cellular Physiology, 234(4), 3526-3537. https://doi.org/10.1002/jcp.26906.