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  • Description:
  • Effect of protein kinase C (PKC) isozyme overexpression on BK‐induced transcriptional activation of COX‐2. Confluent HASM cells (50–60%) were cotransfected with 0.4 μg/well phPES2 (–1432/+59) construct, 4 ng/well pRL‐SV40 internal control vector, and 0.5 μg/well pSRD vectors expressing wild‐type or dominant negative PKC‐α, ‐δ, or ‐ε using FuGene 6 transfection reagent as described in Materials and Methods and were incubated with or without BK (10 μM) for 4 h. Luciferase activities were determined on cell extracts, and relative luciferase activity was calculated as described in Materials and Methods. Each point represents the mean ± SE of eight determinations from two independent experiments. *P<0.05 compared with BK in cells cotransfected with pSRD empty vectors.
  • License:
  • Rights Managed
  • Rights Holder:
  • John Wiley & Sons, Inc.
  • License Rights Holder:
  • © Federation of American Societies for Experimental Biology
  • Asset Type:
  • Image
  • Asset Subtype:
  • Chart/Graph
  • Image Orientation:
  • Landscape
  • Image Dimensions:
  • 1800 x 1146
  • Image File Size:
  • 316 KB
  • Creator:
  • Linhua Pang, Mei Nie, Lisa Corbett, Richard Donnelly, Samuel Gray, Alan J. Knox
  • Credit:
  • Pang, L., Nie, M., Corbett, L., Donnelly, R., Gray, S., & Knox, A. J. (2002). Protein kinase C‐ε mediates bradykinin‐induced cyclooxygenase‐2 expression in human airway smooth muscle cells. The FASEB Journal, 16(11), 1435-1437. https://doi.org/10.1096/fj.02-0169fje.
  • Collection:
  • Keywords:
  • Restrictions:
  • Property Release:
  • No
  • Model Release:
  • No
  • Purchasable:
  • Yes
  • Sensitive Materials:
  • No
  • Article Authors:
  • Linhua Pang, Mei Nie, Lisa Corbett, Richard Donnelly, Samuel Gray, Alan J. Knox
  • Article Copyright Year:
  • 2002
  • Publication Volume:
  • 16
  • Publication Issue:
  • 11
  • Publication Date:
  • 09/01/2002
  • DOI:
  • https://doi.org/10.1096/fj.02-0169fje

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