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Description Metabolic engineering of Pseudomonas with mini‐transposon vectors bearing excisable antibiotic resistances. In this scheme, the selection marker is placed within the transposon vector flanked by...
Article Title: Mini‐transposons in microbial ecology and environmental biotechnology
Publication Title: FEMS Microbiology Ecology -
Description Expression cassettes based on catabolic promoters of Pseudomonas putida. The organization of mini‐Tn5 xylR/Pu, mini‐Tn5 xylS/Pm and mini‐Tn5 nahR/Psal is sketched. The first two contain,...
Article Title: Mini‐transposons in microbial ecology and environmental biotechnology
Publication Title: FEMS Microbiology Ecology -
Description Novel non‐antibiotic selection markers. The different DNA segments encoding the various SfiI/AvrII cassettes are shown on top. The use of the resistance to potassium tellurite encoded by the...
Article Title: Mini‐transposons in microbial ecology and environmental biotechnology
Publication Title: FEMS Microbiology Ecology -
Description Immunomagnetic separation of GMOs labeled with an antigenic surface reporter. Construction of a hybrid LamB‐A6 protein as a surface reporter of promoter activity. The LamB protein of E. coli...
Article Title: Mini‐transposons in microbial ecology and environmental biotechnology
Publication Title: FEMS Microbiology Ecology -
Description Assembly of regulatory cascades with mini‐transposons combined with T7 polymerase and T7 promoters. The figure summarizes the utilization of mini‐Tn5 xylS/Pm::T7pol for constructing strains which...
Article Title: Mini‐transposons in microbial ecology and environmental biotechnology
Publication Title: FEMS Microbiology Ecology -
Description Organization of Tn5‐based transposon vectors and their suicide delivery plasmid. The scheme represents the features common to all constructions, the whole being generally named as the pUT system....
Article Title: Mini‐transposons in microbial ecology and environmental biotechnology
Publication Title: FEMS Microbiology Ecology